A new uranium bioremediation approach using radio-tolerant Deinococcus radiodurans biofilm
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Date
2019-10
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Academy of Sciences
Abstract
Deinococcus radiodurans is the most radiation-tolerant organism ever known. It has gained importance in recent years as apotential candidate for bioremediation of heavy metals, especially the radioactive type. This study investigates the efficiency of a recombinant D. radiodurans (DR1-bf?) strain with an ability to form biofilm for uranium remediation. Themodified Arsenazo III dye method was used to estimate the uranium concentration. Uranyl nitrate aqueous solution wasgenerated during the operation of nuclear fuel reprocessing. The D. radiodurans biofilm (DR1-bf?) grown in the presenceof 20 mM Ca2? showed remarkable ability of uranyl ion removal. DR1-bf? (?Ca2?) biofilm removed *75±2% of1000 mg/L uranium within 30 min post-treatment from uranyl nitrate aqueous solution. Uranium removal rate was alsofound to be directly proportional to biofilm age. This study discusses the ability of D. radiodurans biofilm in uraniumremoval.
Description
Keywords
Arsenazo, biofilm, bioremediation, Deinococcus radiodurans, radio-tolerance, uranium
Citation
Manobala T, Shukla Sudhir K, Rao T Subba, Kumar M Dharmendira. A new uranium bioremediation approach using radio-tolerant Deinococcus radiodurans biofilm. Journal of Biosciences. 2019 Oct; 44(5): 1-9