Gonadotropin releasing hormone in first trimester human placenta: Isolation, partial characterisation and in vitro biosynthesis.
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Date
1986-12
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Abstract
Using a specific radioimmunoassay for gonadotropin releasing hormone, the
presence of gonadotropin releasing hormone like material in the first trimester human
placenta has been demonstrated. The material has been partially characterized using
carboxy methyl cellulose chromatography, high pressure gel permeation chromatography
and reverse phase C18 high pressure liquid chromatographic analysis. Analysis for
bioactivity revealed that placental gonadotropin releasing hormone is much more active
than synthetic gonadotropin releasing hormone in in vitro rat pituitary lutinising hormone
release assay. In vitro biosynthetic studies using labelled precursors and immunoaffinity
chromatography indicated that first trimester human placenta synthesizes gonadotropin
releasing hormone like material.
Description
Keywords
Placenta, gonadotropin releasing hormone, receptor assay, biosynthesis, immunoaffinity
Citation
Mathialagan N, Rao A Jagannadha. Gonadotropin releasing hormone in first trimester human placenta: Isolation, partial characterisation and in vitro biosynthesis. Journal of Biosciences. 1986 Dec; 10(4): 429-441.