Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease.

Simple item page
dc.contributor.authorSasikala, Mitnala
dc.contributor.authorReddy, D Nageshwar
dc.contributor.authorPratap, Nitesh
dc.contributor.authorSharma, Sanjeev Kumar
dc.contributor.authorReddy, P Balkumar
dc.contributor.authorSekaran, Anuradha
dc.contributor.authorBanerjee, Rupa
dc.contributor.authorReddy, D Bhaskara
dc.date.accessioned2012-09-18T03:14:02Z
dc.date.available2012-09-18T03:14:02Z
dc.date.issued2009-09
dc.description.abstractBackground and Objective The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn’s disease (CD) remains controversial, because of conflicting reports demonstrating the presence of MAP-specific insertion sequence from intestinal biopsy tissues of patients clinically diagnosed for the disease. The present study was carried out to investigate the presence of MAP DNA in the intestinal tissues of CD patients to ascertain the relevance of MAP in Indian patients with CD. Methods Patients diagnosed as CD at our institute were recruited. Healthy individuals without inflammatory bowel disease served as controls. Mucosal biopsy specimens were collected from ileum and colon in duplicates and subjected to histopathological examination and polymerase chain reaction (PCR) amplification. Total DNA (81 CD patients, 85 healthy individuals) and total RNA (12 CD patients, 12 healthy individuals) isolated from tissue specimens was used for amplification of MAP-specific IS900 by nested PCR. Results MAP-specific IS900 DNA and RNA could not be detected by nested PCR in the intestinal tissues of any patient with CD. Conclusion Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.en_US
dc.identifier.citationSasikala Mitnala, Reddy D Nageshwar, Pratap Nitesh, Sharma Sanjeev Kumar, Reddy P Balkumar, Sekaran Anuradha, Banerjee Rupa, Reddy D Bhaskara. Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease. Indian Journal of Gastroenterology. 2009 Sept-Oct; 28(5): 169-174.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/141447
dc.language.isoenen_US
dc.source.urihttps://www.indianjgastro.com/IJG_pdf/sept2009/September_2.pdfen_US
dc.subjectInflammatory bowel diseaseen_US
dc.subjectMAP-specific IS900en_US
dc.subjectMycobacterium avium ss paratuberculosisen_US
dc.titleAbsence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn’s disease.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijg2009v28n5p169.pdf
Size:
841.38 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Gastroenterology