Alteration of the structure of the Escherichia coli ribosomes on treatment with Fab fragment of immunoglobulin raised against the ribosomes.
Loading...
Date
1979-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Rabbits were immunised against Escherichia coli ribosomes and the
partially purified immunoglobulin G fraction had maximum ability to precipitate
the ribosomes as well as the extracted ribosomal proteins. By digestion of immunoglobulin
G with papain, monovalent Fab fragments were produced. The 70 S ribosome
and its subunits (50 S and 30 S) were separately treated with Fab and then tested
in the kinetic assay of degradation of ribosomes by ribonuclease I at various Mg2+
concentrations. Treated ribosomes and their subunits were degraded at faster rates
than the nontreated ones; the rates in both the control and the treated cases were
dependent on the concentration of Mg2+. These results indicate the unfolding of
the structure of the ribosome on treatment with antibody fragments, which may be
due to the weakening of the interaction between rRNAs and ribosomal proteins.
Description
Keywords
Ribosomes, ribosomal subunits, antibody, immunoglobulin, Fab, ribonuclease I
Citation
Das M, Burma D P. Alteration of the structure of the Escherichia coli ribosomes on treatment with Fab fragment of immunoglobulin raised against the ribosomes. Journal of Biosciences. 1979 Mar; 1(1): 83-89.