Polymorphisms in the apolipoprotein B-100 gene: association with plasma lipid concentration and coronary artery disease.

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dc.contributor.authorPuri, Ratna Duaen_US
dc.contributor.authorTewari, Satyendraen_US
dc.contributor.authorSinha, Nakulen_US
dc.contributor.authorRamesh, Ven_US
dc.contributor.authorKhan, Faisalen_US
dc.contributor.authorSingh, Vivek Pen_US
dc.contributor.authorAgrawal, Surakshaen_US
dc.date.accessioned2003-01-23en_US
dc.date.accessioned2009-05-27T04:24:59Z
dc.date.available2003-01-23en_US
dc.date.available2009-05-27T04:24:59Z
dc.date.issued2003-01-23en_US
dc.description.abstractBACKGROUND: The aim of this study was to investigate the association of apolipoprotein B gene polymorphisms with coronary artery disease and lipid levels in Indians. METHODS AND RESULTS: One hundred patients of angiographically proven atherosclerotic coronary artery disease and one hundred age- and sex-matched control subjects (treadmill negative) were included in the study. Serum lipids including cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, very low-density lipoprotein, and apolipoprotein B were analyzed. Genomic DNA was extracted and the apolipoprotein B 3' hypervariable region amplified by polymerase chain reaction. Regions carrying Xba1, EcoR1, and Msp1 restriction sites present in the apolipoprotein B gene were amplified and digested separately by the respective enzymes. Restriction fragment length polymorphism analysis showed that EcoR1 with the R+/R+ genotype was significantly more common in patients with coronary artery disease. Overall, the genotypes EcoR1+/+, Msp1+/+, Xba1+/+ and Eco R1+/+ Msp1+/-, Xba1-/- were significantly more common in patients as compared to controls (p<0.05). When gene polymorphisms were compared with lipid abnormalities, the genotypes EcoR1+/+, Xba1-/-, and Msp1+/+ were more frequent in patients with elevated apolipoprotein B and very low-density lipoprotein levels. On the other hand, these genotypes were less common in patients with increased total cholesterol and low-density lipoprotein levels. When we studied the individual alleles of the variable number of tandem repeats region, we observed that allele 34 was significantly increased in patients with coronary artery disease as compared to controls. Allele 36 was present with a frequency of 1% in controls while it was totally absent in patients. CONCLUSIONS: This study identifies the apolipoprotein B gene polymorphism associated with coronary artery disease. An association between apolipoprotein B gene polymorphisms and elevated apolipoprotein B and very low-density lipoprotein levels was observed. However, there was no positive association with other elevated lipid levels in North Indians from Uttar Pradesh.en_US
dc.description.affiliationDepartment of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow.en_US
dc.identifier.citationPuri RD, Tewari S, Sinha N, Ramesh V, Khan F, Singh VP, Agrawal S. Polymorphisms in the apolipoprotein B-100 gene: association with plasma lipid concentration and coronary artery disease. Indian Heart Journal. 2003 Jan-Feb; 55(1): 60-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/4894
dc.language.isoengen_US
dc.source.urihttps://indianheartjournal.comen_US
dc.subject.meshApolipoprotein B-100en_US
dc.subject.meshApolipoproteins B --geneticsen_US
dc.subject.meshCoronary Artery Disease --geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHumansen_US
dc.subject.meshLipoproteins --blooden_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMinisatellite Repeatsen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.titlePolymorphisms in the apolipoprotein B-100 gene: association with plasma lipid concentration and coronary artery disease.en_US
dc.typeJournal Articleen_US
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