In vitro resistance of leukaemic blasts to prednisolone in bcr-abl positive childhood acute lymphoblastic leukaemia.

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dc.contributor.authorGupta, Mamtaen_US
dc.contributor.authorKumar, Anilen_US
dc.contributor.authorDabadghao, Sunilen_US
dc.date.accessioned2002-12-17en_US
dc.date.accessioned2009-05-27T07:55:07Z
dc.date.available2002-12-17en_US
dc.date.available2009-05-27T07:55:07Z
dc.date.issued2002-12-17en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Although the outcome of children with acute lymphoblastic leukaemia (ALL) has improved dramatically over the last decade, some children still fare poorly and relapses are seen. The sensitivity of leukaemic cells to corticosteroids has emerged as an important prognostic factor in ALL. The t(9,22) translocation, resulting in the bcr-abl fusion gene, is a non-random translocation found in B-lineage acute lymphoblastic leukaemia. It is also known to be an independent poor prognostic factor for long-term disease free survival. We studied the association between the presence of bcr-abl fusion gene and in vitro prednisolone resistance in children with B-lineage acute lymphoblastic leukaemia at diagnosis. METHODS: A total of 23 children (aged 1-16 yr, median age: 12 yr) with B-lineage acute lymphoblastic leukaemia at diagnosis were included in the study. The presence of bcr-abl fusion gene was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and the in-vitro resistance to prednisolone was measured by short term colorimetric methyl thiazol tetrazolium (MTT) assay. RESULTS: A median LD50 (lethal dose for 50% cells) for prednisolone in bcr-abl positive children (n=7) was 1.6 mg/ml (range: 0.25-5.0 mg/ml) and that of bcr-abl negative children (n=16) was 0.35 mg/ml (range 0.62-1.0 mg/ml). The median LD50 for prednisolone differed significantly between the bcr-abl positive and negative groups of children with acute lymphoblastic leukaemia (P<0.005). INTERPRETATION & CONCLUSION: This is probably the first report to show that leukaemic blasts of bcr-abl positive children with ALL are about four-fold resistant to prednisolone as compared to blasts from bcr-abl negative children. This suggests that one of the reasons for the poor prognosis of bcr-abl positive ALL could be a lower steroid sensitivity.en_US
dc.description.affiliationDepartment of Immunology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.en_US
dc.identifier.citationGupta M, Kumar A, Dabadghao S. In vitro resistance of leukaemic blasts to prednisolone in bcr-abl positive childhood acute lymphoblastic leukaemia. Indian Journal of Medical Research. 2002 Dec; 116(): 268-72en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/22563
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAntineoplastic Agents, Hormonal --pharmacologyen_US
dc.subject.meshBurkitt Lymphoma --drug therapyen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshCytotoxicity Tests, Immunologicen_US
dc.subject.meshDrug Resistance, Neoplasmen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, ablen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshLymphocytes --cytologyen_US
dc.subject.meshMaleen_US
dc.subject.meshPhiladelphia Chromosomeen_US
dc.subject.meshPrednisolone --pharmacologyen_US
dc.subject.meshPrognosisen_US
dc.titleIn vitro resistance of leukaemic blasts to prednisolone in bcr-abl positive childhood acute lymphoblastic leukaemia.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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