Molecular cloning and expression of Salmonella typhi flagellin: characterization of 52 kDa specific antigen of S. typhi.

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dc.contributor.authorSukosol, Ten_US
dc.contributor.authorSarasombath, Sen_US
dc.contributor.authorMongkolsuk, Sen_US
dc.contributor.authorSongsivilai, Sen_US
dc.contributor.authorChaiyaroj, Sen_US
dc.contributor.authorPongsunk, Sen_US
dc.contributor.authorEkpo, Pen_US
dc.date.accessioned2009-05-27T17:14:29Z
dc.date.available2009-05-27T17:14:29Z
dc.date.issued1993-06-01en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractWe previously reported monoclonal antibodies (MAbs) specific to S. typhi 52 kDa antigen which do not cross react with related protein antigens from 11 bacteria causing enteric fever and enteric fever-like illness. Using the combination of these specific MAbs and recombinant DNA technology, expression plasmids containing the antigen gene producing substantial amount of the S. typhi protein antigen have been established. Plasmid pSKM-T7 containing the specific 52 kDa antigen gene was cloned and the antigen expressed was detectable by immunoblotting using specific mAbs. The complete nucleotide sequence of this gene was compared with other bacterial sequences and found to be highly homologous with the flagellin gene H1-d of S. muenchen except in the hypervariable region in the central portion. The specific 52 kDa antigen of S. typhi detected by our MAbs is thus a flagellin.en_US
dc.description.affiliationDepartment of Immunology, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand.en_US
dc.identifier.citationSukosol T, Sarasombath S, Mongkolsuk S, Songsivilai S, Chaiyaroj S, Pongsunk S, Ekpo P. Molecular cloning and expression of Salmonella typhi flagellin: characterization of 52 kDa specific antigen of S. typhi. Asian Pacific Journal of Allergy and Immunology. 1993 Jun; 11(1): 57-69en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36760
dc.language.isoengen_US
dc.subject.meshAntibodies, Monoclonal --immunologyen_US
dc.subject.meshAntigens, Bacterial --geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA, Bacterial --geneticsen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFlagellin --geneticsen_US
dc.subject.meshGene Expression Regulation, Bacterialen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshSalmonella typhi --geneticsen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.titleMolecular cloning and expression of Salmonella typhi flagellin: characterization of 52 kDa specific antigen of S. typhi.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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