Cyclospora cayetanensis: oocyst characteristics and excystation.
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Date
2002-03-16
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Abstract
In Thailand in 1999-2000, Cyclospora oocysts from two HIV-infected patients and one patient with prolonged diarrhea were detected by formalin-ether concentration technique. Sporulation was performed by mixing stool samples in 2.5% potassium dichromate solution, sporulated oocysts were then treated with various solutions before mechanical rupturing in order to establish excystation, fewer than 10% of the sporulated oocysts could be excysted. Our techniques provided more details of the characteristic appearance of sporocysts and sporozoites within the oocysts (DMSO-modified acid-fast technique with our modification).
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Citation
Siripanth C, Phraevanich R, Suphadtanaphongs W, Thima N, Radomyos P. Cyclospora cayetanensis: oocyst characteristics and excystation. The Southeast Asian Journal of Tropical Medicine and Public Health. 2002 ; 33 Suppl 3(): 45-8