Effect of N-bromosuccinimide-modification of tyrosine side chains of cardiotoxin II of the Indian cobra on biological activity.

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dc.contributor.authorShashidharan, P
dc.contributor.authorRamachandran, L K
dc.date.accessioned2015-07-24T09:06:20Z
dc.date.available2015-07-24T09:06:20Z
dc.date.issued1987-03
dc.description.abstractThe essential role of tyrosine residue(s) of cardiotoxin II in the biological activity of the toxin was evaluated using N-bromosuccinimide. N-bromosuccinimide effected oxidation of the tyrosine residues in cardiotoxin II with enhancement in absorbance at 260 nm. The influence of various solvent media such as acetate-formate buffer (pH 4·0),0·01 Ν H2SO4 (pH 2·0) and Tris-HCl buffer (pH 8·5) on oxidation of tyrosine residues was exa mined. In comparison with 0·01 Ν H2S O4, acetate-formate buffer could prevent secondary oxidations as revealed by lower consumption of oxidant, N-bromosuccinimide, to achieve oxidation. In Tris-HCl buffer oxidation of tyrosine did not take place effectively. N-iodosuccinimide caused only limited oxidation as evident from minor increase in absorbance at 260 nm. N-chlorosuccinimide was completely ineffective. Oxidation of cardiotoxin II with 3·75 equivalents of N-bromosuccinimide tyrosine residue led to complete loss of lethal activity. However, the derivative retained the ability to protect bacterial protoplasts from lysis in solutions of low tonicity. Unlike cardiotoxin II oxidized with N-chlorosuccinimide (50 equivalents/mol of toxin) which retained lethal activity as well as the ability to protect protoplasts from lysis, performic acid-oxidized toxin had lost both the activities.en_US
dc.identifier.citationShashidharan P, Ramachandran L K. Effect of N-bromosuccinimide-modification of tyrosine side chains of cardiotoxin II of the Indian cobra on biological activity. Journal of Biosciences. 1987 Mar; 11(1-4): 287-297.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/160527
dc.language.isoenen_US
dc.source.urihttps://www.ias.ac.in/jarch/jbiosci/11/287-297.pdfen_US
dc.subjectCardiotoxin IIen_US
dc.subjectNaja najaen_US
dc.subjectN-bromosuccinimide oxidationen_US
dc.subjectessential tyrosineen_US
dc.subjectprotoplast stabilizationen_US
dc.titleEffect of N-bromosuccinimide-modification of tyrosine side chains of cardiotoxin II of the Indian cobra on biological activity.en_US
dc.typeArticleen_US
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