Baculovirus vector-mediated expression of heterologous genes in insect cells.
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Date
1994-12
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Abstract
The baculovirus expression system employing Autagrapha californica nuclear
polyhidrosis virus and Spodoptera frugiperda insect cells in culture has proved very popular
for high level expression of heterologous genes: In this system, transcription of the foreign
gene is usually driven by the hyperactive and temporally regulated polyhedrin gene promoter.
Replacement of the polyhedrin gene, which encodes a 29-kDa occlusion protein (non-essential
for viral replication), with a gene of interest leads to an occlusion negative phenotype
which serves as a visual marker to select for recombinant viruses. Simultaneous expression
of multiple genes can also be achieved. The heterologous proteins synthesized in this
system are antigenically, immunologically and functionally identical in most respects to
their native counterparts. This mini-review will aim at summarizing the potentials and
utility of the baculovirus expression vector system and will address some important questions
relating to the biology of this system.
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Keywords
Baculovirus, heterologous gene, Autagrapha californica, Spodoptera frugiperda, insect cells
Citation
Sridhar P, Awasthi A K, Azim C A, Burma S, Habib S, Jain A, Mukherjee B, Ranjan B, Hasnain Seyed E. Baculovirus vector-mediated expression of heterologous genes in insect cells. Journal of Biosciences. 1994 Dec; 19(5): 603-614.