Investigations into size heterogeneity of the alpha B-crystallin mRNA.

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dc.contributor.authorSrinivasan, Aen_US
dc.contributor.authorBhat, S Pen_US
dc.date.accessioned1994-08-01en_US
dc.date.accessioned2009-05-27T09:24:37Z
dc.date.available1994-08-01en_US
dc.date.available2009-05-27T09:24:37Z
dc.date.issued1994-08-01en_US
dc.description.abstractalpha B-crystallin is expressed in a variety of developmental, physiological and pathological conditions in a number of tissues. Based on northern blots of total RNA, the existence of at least two size classes of alpha B-crystallin mRNAs has been reported, one smaller predominant species, 0.8-0.9 kb, and the other 1.2 to 1.4 kb. This heterogeneity has been attributed to alternative upstream transcriptional initiation. We have investigated the origin of the size heterogeneity of alpha B-crystallin mRNA by using 5'-upstream-, coding- and 3'-untranslated-region probes in RNAse protection and northern blot assays. RNAse protection assays indicate that there is only one predominant initiation site as previously reported and that the second polyadenylation signal is not used in the rat gene. Importantly, northern blot data obtained with coding region-only probe shows that the size of alpha B mRNA detected in the heart and the lens is similar (0.78 kb) in poly A+ as well as in total RNA. On the other hand, in the brain and in the lung, the larger hybridizing species (1.05 kb and 1.22 kb respectively) seen in total RNA are not detected in poly A+RNA which shows a 0.95 kb species in both tissues. The 5' upstream probe (-1 to -499) produces weak hybridization patterns in the brain and the lung, similar to those obtained with coding region-only probe. The 5' probe did not show hybridization in the heart and the lens RNAs. These data suggest that upstream initiations represent a minor population of transcripts and that higher size transcripts (about 500 bp larger) actually represent non-polyadenylated RNAs that may not contribute to the generation of the actual gene product.en_US
dc.description.affiliationJules Stein Eye Institute, University of California, Los Angeles 90024-7008.en_US
dc.identifier.citationSrinivasan A, Bhat SP. Investigations into size heterogeneity of the alpha B-crystallin mRNA. Indian Journal of Biochemistry & Biophysics. 1994 Aug; 31(4): 351-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27421
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCrystallins --geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshRNA, Messenger --analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleInvestigations into size heterogeneity of the alpha B-crystallin mRNA.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, P.H.S.en_US
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