Bioconjugate-loaded solid lipid nanoparticles for enhanced anticancer drug delivery to brain cancer cells: An in vitro evaluation

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dc.contributor.authorJain, Priyankaen_US
dc.contributor.authorPandey, Vikasen_US
dc.contributor.authorSoni, Vandanaen_US
dc.date.accessioned2023-08-19T04:52:24Z
dc.date.available2023-08-19T04:52:24Z
dc.date.issued2022-07
dc.description.abstractBackground & objectives: The treatment of brain cancer is still challenging for an oncologist due to the presence of the blood-brain barrier (BBB) which inhibits the entry of more than 98 per cent of drugs used during the treatment of brain disease. The cytotoxic drugs used in chemotherapy for brain cancer treatment also affect the normal cells due to lack of targeting. Therefore, the objective of the study was to develop tween 80-coated solid lipid nanoparticles (SLNs) loaded with folic acid-doxorubicin (FAD) conjugate for site-specific drug delivery to brain cancer cells. Methods: The FAD conjugate was synthesized by the conjugation of folic acid with doxorubicin and characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. SLNs loaded with FAD were prepared by the solvent injection method. The SLNs were characterized by the particle size, zeta potential, surface morphology, entrapment efficiency, etc. Results: The average particle size of FAD conjugate-loaded SLNs (SLN-C) was found to be 220.4±2.2 nm, with 36.2±0.6 per cent entrapment efficiency. The cytotoxicity and cellular uptake were determined on U87 MG cell lines. Half maximal inhibitory concentration value of the SLN-C was found to be 2.5 ?g/ml, which confirmed the high antitumour activity against brain cancer cells. Interpretation & conclusions: The cell line studies confirmed the cytotoxicity and internalization of SLN-C in U87 MG brain cancer cells. The results confirmed that tween 80-coated SLNs have the potential to deliver the doxorubicin selectively in the brain cancer cells.en_US
dc.identifier.affiliationsDepartment of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar, Madhya Pradesh, Indiaen_US
dc.identifier.citationJain Priyanka, Pandey Vikas, Soni Vandana. Bioconjugate-loaded solid lipid nanoparticles for enhanced anticancer drug delivery to brain cancer cells: An in vitro evaluation. Indian Journal of Medical Research. 2022 Jul; 156(1): 139-148en_US
dc.identifier.issn0971-5916
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223640
dc.languageenen_US
dc.publisherWolters Kluwer – Medknowen_US
dc.relation.issuenumber1en_US
dc.relation.volume156en_US
dc.source.urihttps://doi.org/10.4103/ijmr.IJMR_514_19en_US
dc.subjectBrain canceren_US
dc.subjectconjugateen_US
dc.subjectdoxorubicinen_US
dc.subjectfolic aciden_US
dc.subjectsolid lipid nanoparticlesen_US
dc.titleBioconjugate-loaded solid lipid nanoparticles for enhanced anticancer drug delivery to brain cancer cells: An in vitro evaluationen_US
dc.typeJournal Articleen_US
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