In vitro characterization of repair synthesis initiated by T4 endonuclease V on a synthetic DNA substrate.

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dc.contributor.authorSibghat-Ullah,en_US
dc.contributor.authorSancar, Aen_US
dc.date.accessioned1992-06-01en_US
dc.date.accessioned2009-05-27T09:22:21Z
dc.date.available1992-06-01en_US
dc.date.available2009-05-27T09:22:21Z
dc.date.issued1992-06-01en_US
dc.description.abstractThe size of the repair patch produced by E. coli DNA polymerase (Pol I) following the removal of a pyrimidine dimer from DNA in response to the nicking activity of T4 endonuclease (T4 endo V) was determined. A 48-bp DNA containing a pyrimidine dimer at a defined location was labelled in the damaged strand and incubated with T4 endo V and E. coli endonuclease IV. Subsequently, DNA synthesis by DNA Pol I was carried out in the presence of four dNTPs, ATP and DNA ligase. Analysis of the reaction products on a sequencing gel revealed a ladder of only 4-oligonucleotides, 1-4 nucleotides greater in length than the fragment generated by the combined nicking activities of T4 endo V and E. coli endonuclease IV. Thus we conclude that the in vitro repair patch size of T4 endo V is 4 nucleotides and that in some cases the repaired DNA is not ligated.en_US
dc.description.affiliationDepartment of Biochemistry and Biophysics, University of North Carolina, School of Medicine, Chapel Hill 27599.en_US
dc.identifier.citationSibghat-Ullah , Sancar A. In vitro characterization of repair synthesis initiated by T4 endonuclease V on a synthetic DNA substrate. Indian Journal of Biochemistry & Biophysics. 1992 Jun; 29(3): 227-30en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27303
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDNA --metabolismen_US
dc.subject.meshDNA Polymerase I --metabolismen_US
dc.subject.meshDNA Repairen_US
dc.subject.meshDNA Replicationen_US
dc.subject.meshDeoxyribonuclease (Pyrimidine Dimer)en_US
dc.subject.meshEndodeoxyribonucleases --metabolismen_US
dc.subject.meshEscherichia coli --enzymologyen_US
dc.subject.meshModels, Structuralen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNucleic Acid Conformationen_US
dc.subject.meshOligodeoxyribonucleotidesen_US
dc.subject.meshSubstrate Specificityen_US
dc.subject.meshT-Phages --enzymologyen_US
dc.subject.meshViral Proteinsen_US
dc.titleIn vitro characterization of repair synthesis initiated by T4 endonuclease V on a synthetic DNA substrate.en_US
dc.typeJournal Articleen_US
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