Prevalence of dengue virus in Aedes mosquitoes during dry season by semi-nested reverse transcriptase-polymerase chain reaction (semi-nested RT-PCR).

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dc.contributor.authorTuksinvaracharn, Ruppornen_US
dc.contributor.authorTanayapong, Pongsakornen_US
dc.contributor.authorPongrattanaman, Saravechen_US
dc.contributor.authorHansasuta, Pokrathen_US
dc.contributor.authorBhattarakosol, Parvapanen_US
dc.contributor.authorSiriyasatien, Padeten_US
dc.date.accessioned2009-05-27T18:02:45Z
dc.date.available2009-05-27T18:02:45Z
dc.date.issued2004-09-09en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractDengue hemorrhagic fever remains a major health concern in Thailand. Much effort has focused on the prevention and control of the disease. Detection of dengue virus infection rate in mosquitoes would evaluate dengue control programs and predict the epidemics of dengue hemorrhagic fever. To determine dengue virus infection rate in mosquitoes by Semi-nested RT-PCR. A total of 400 mosquitoes were collected from Rom Kao Community representing a crowded community and another 9 non-crowded communities in Bangkok. Mosquitoes were then divided into 40 pools, each contained 10 mosquitoes. A total of 391 Aedes aegypti and 9 Aedes albopictus were screened for dengue virus. The mosquito infection rate in the Rom Klao community was 5% of the mosquito pool equal to that found in non-crowded communities. Both groups were found to have dengue virus serotype 3. The present study suggests a circulation of dengue virus serotype 3 in both crowded and non-crowed communities, the infection rates of which are indifferent during the dry season.en_US
dc.description.affiliationDepartment of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.en_US
dc.identifier.citationTuksinvaracharn R, Tanayapong P, Pongrattanaman S, Hansasuta P, Bhattarakosol P, Siriyasatien P. Prevalence of dengue virus in Aedes mosquitoes during dry season by semi-nested reverse transcriptase-polymerase chain reaction (semi-nested RT-PCR). Journal of the Medical Association of Thailand. 2004 Sep; 87 Suppl 2(): S129-33en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/38341
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAedes --virologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDengue Virus --geneticsen_US
dc.subject.meshElectrophoresis, Agar Gelen_US
dc.subject.meshRNA, Viral --isolation & purificationen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction --methodsen_US
dc.subject.meshSeasonsen_US
dc.subject.meshThailanden_US
dc.titlePrevalence of dengue virus in Aedes mosquitoes during dry season by semi-nested reverse transcriptase-polymerase chain reaction (semi-nested RT-PCR).en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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