Alteration of gastric microbiota in Helicobacter pylori- infected individuals suffering from gastroesophageal reflux disease, duodenal ulcer, and gastritis

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dc.contributor.authorSharma, Pen_US
dc.contributor.authorSingh, Sen_US
dc.contributor.authorSingh, AVen_US
dc.contributor.authorDas, Ken_US
dc.contributor.authorBhaskar, Yen_US
dc.contributor.authorGoel, Ien_US
dc.contributor.authorSingh, Hen_US
dc.contributor.authorDas, Ren_US
dc.date.accessioned2025-06-19T05:55:13Z
dc.date.available2025-06-19T05:55:13Z
dc.date.issued2025-03
dc.description.abstractBackground/Aim: Helicobacter pylori (H. pylori) colonization affects the gastric microbiome, causing gastrointestinal (GI) diseases. Modern sequencing technology provides insights into GI microbe interaction with H. pylori and their metabolic pathways in causing GI diseases. We aim to compare the gastric microbiota alteration due to H. pylori infection in patients suffering from GI diseases. Materials and Methods: Genomic DNA were isolated from gastric antrum tissue from 37 H.pylori?infected patients diagnosed with GERD, duodenal ulcers, and gastritis. We conducted the genomic library preparation and sequencing of the amplified product using 16S rRNA NGS analysis. Using microbiome analyst tool diversity analysis, random forest analysis and ANOVA were conducted to find out the comparison of microbial abundance. We have also conducted functional pathway prediction analysis using PICRUSt. Results: Metagenomic analysis shows high bacterial diversity in H. pylori?positive gastritis patients. Streptococcus infantis and Neisseria subflava were significantly higher in duodenal ulcer (DU) and gastritis groups. Acinetobacter lwoffii and Helicobacter pullorum were significantly high in the gastritis group only. The functional metabolic pathway analyses revealed that gastroesophageal reflux disease (GERD) samples were significantly enriched with the energy metabolism and xenobiotic biodegradation and metabolism pathways, whereas fructose?1,6?bisphosphatase III was found less in gastritis and DU groups. Conclusion: There is a difference in microbiota composition in different disease outcomes. We found positive association between microbial diversity and H. pylori in gastritis group only, whereas negative association was found in DU and GERD groups. The functional metabolic pathway analysis revealed significant differences in various disease outcomes.en_US
dc.identifier.affiliationsCenter for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.affiliationsCenter for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.affiliationsCenter for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.affiliationsDepartment of Gastroenterology, Yashoda Super Specialty Hospital, Ghaziabad, Uttar Pradesh; Department of Gastroenterology, Max Super?Specialty Hospital, Vaishali, Ghaziabad, Uttar Pradeshen_US
dc.identifier.affiliationsICMR?AIIMS Computational Genomics Centre, Division of Biomedical Informatics, Indian Council of Medical Research (ICMR), New Delhien_US
dc.identifier.affiliationsICMR?AIIMS Computational Genomics Centre, Division of Biomedical Informatics, Indian Council of Medical Research (ICMR), New Delhien_US
dc.identifier.affiliationsDepartment of Psychiatry, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsCenter for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradeshen_US
dc.identifier.citationSharma P, Singh S, Singh AV, Das K, Bhaskar Y, Goel I, Singh H, Das R. Alteration of gastric microbiota in Helicobacter pylori- infected individuals suffering from gastroesophageal reflux disease, duodenal ulcer, and gastritis. Indian Journal of Pathology & Microbiology. 2025 Mar; 68(1): 61-68en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/249966
dc.languageenen_US
dc.publisherWolters Kluwer - Medknowen_US
dc.relation.issuenumber1en_US
dc.relation.volume68en_US
dc.source.urihttps://doi.org/10.4103/ijpm.ijpm_1015_23en_US
dc.subjectDysbiosisen_US
dc.subjectfunctional pathwaysen_US
dc.subjectgastric microbiotaen_US
dc.subjecthelicobacter pullorumen_US
dc.subjecthelicobacter pylorien_US
dc.titleAlteration of gastric microbiota in Helicobacter pylori- infected individuals suffering from gastroesophageal reflux disease, duodenal ulcer, and gastritisen_US
dc.typeJournal Articleen_US
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