Recombinant human erythropoietin (rhEPO) preconditioning on nuclear factor-kappa B (NF-kB) activation & proinflammatory cytokines induced by myocardial ischaemia-reperfusion.
dc.contributor.author | Liu, Xiaoming | en_US |
dc.contributor.author | Shen, Jinchun | en_US |
dc.contributor.author | Jin, Yi | en_US |
dc.contributor.author | Duan, Manlin | en_US |
dc.contributor.author | Xu, Jianguo | en_US |
dc.date.accessioned | 2006-09-07 | en_US |
dc.date.accessioned | 2009-05-27T08:31:21Z | |
dc.date.available | 2006-09-07 | en_US |
dc.date.available | 2009-05-27T08:31:21Z | |
dc.date.issued | 2006-09-07 | en_US |
dc.description.abstract | BACKGROUND & OBJECTIVES: Erythropoietin (EPO), originally identified for its critical hormonal role in promoting erythrocyte survival and differentiation, has shown to a protective effect in myocardial ischaemia-reperfusion (I-R) injury in animal model. However, the precise mechanisms remain unclear. The objective of this study was to determine the roles of nuclear factor-kappa B (NF-kB) and associated cytokines induced by I-R in the cardioprotection by recombinant human erythropoietin (rhEPO). Morphopathological observations were also made on the ultrastructure of myocardial tissue. METHODS: Myocardial I-R rat model was established by 30 min ligation of left descending coronary and 3 h reperfusion. RhEPO or saline solution was intraperitoneally injected 24 h before I-R insult. The infarct sizes were measured by triphenyltetrazolium chloride (TTC)-Evans blue technique and ultrastructural organizations were observed by a transmission electron microscope. Tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-10 concentrations were analyzed by enzyme-linked immunosorbance assays and NF-kB by electrophoretic mobility shift assay. TNF-alpha and IL-6 mRNA expression were studied by the reverse-transcription polymerase chain reaction (RT-PCR). RESULTS: A single bolus injection of 5,000 units/kg of rhEPO 24 h before insult remarkably reduced infarct size and improved ultrastructural organization of I-R myocardium. It greatly suppressed TNF-alpha and IL-6 expression, but enhanced IL-10 production. It modestly activated NF-kB before I-R insult and markedly attenuated subsequent NF-kB activation during sustained I-R. INTERPRETATION & CONCLUSION: The suppression of proinflammatory cytokines expression may act by inhibiting NF-kB activation during I-R, but not by induction of IL-10. This might be one of the molecular mechanisms of rhEPO in cardioprotection. In addition, NF-kB was suggested to play a dual role in cardioprotective effects of rhEPO. | en_US |
dc.description.affiliation | Department of Anaesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China. | en_US |
dc.identifier.citation | Liu X, Shen J, Jin Y, Duan M, Xu J. Recombinant human erythropoietin (rhEPO) preconditioning on nuclear factor-kappa B (NF-kB) activation & proinflammatory cytokines induced by myocardial ischaemia-reperfusion. Indian Journal of Medical Research. 2006 Sep; 124(3): 343-54 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/24741 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://icmr.nic.in/ijmr/ijmr.htm | en_US |
dc.subject.mesh | Analysis of Variance | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cytokines --metabolism | en_US |
dc.subject.mesh | DNA Primers | en_US |
dc.subject.mesh | Electrophoretic Mobility Shift Assay | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_US |
dc.subject.mesh | Erythropoietin, Recombinant --metabolism | en_US |
dc.subject.mesh | Ischemic Preconditioning, Myocardial --methods | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Microscopy, Electron, Transmission | en_US |
dc.subject.mesh | Myocardial Reperfusion Injury --metabolism | en_US |
dc.subject.mesh | Myocardium --ultrastructure | en_US |
dc.subject.mesh | NF-kappa B --metabolism | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.title | Recombinant human erythropoietin (rhEPO) preconditioning on nuclear factor-kappa B (NF-kB) activation & proinflammatory cytokines induced by myocardial ischaemia-reperfusion. | en_US |
dc.type | Comparative Study | en_US |
dc.type | Journal Article | en_US |
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