Screening Of In Vitro Antiviral Activity Of Purified Terpenoid Extracts Of Selected Sea Weeds Against Chikungunya Virus

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dc.contributor.authorSumayya , S. S.en_US
dc.contributor.authorLubaina, A. S.en_US
dc.contributor.authorMurugan, K.en_US
dc.date.accessioned2020-10-16T07:51:18Z
dc.date.available2020-10-16T07:51:18Z
dc.date.issued2020-07
dc.description.abstractCurrently, the search of novel phytochemicals with unique biological potentialities is a pre-requisite for the designing ideal drugs for the human kind. Sea weeds are bioresources with a broad spectrum of medicinal properties with minimal side effects. Kerala, the Southern state of India reported high incidence of Chikungunya virus (CHIKV) infections in the last several tears. No specific virucidal therapy or effective vaccines are available. This emphasizes the need of searching for phytochemicals as drugs with less cost and more effective. Therefore, an attempt was made in screening purified terpenoid extracts of selected sea weeds as anti-CHIKV potential. In this study the terpenoids composition from the red algae Hypnea musciformis, Kappaphycus alvarezii and Gracillaria dura were identified and analyzed by thin layer chromatography and Gas chromatography- Mass spectrum. The methanolic extract of seaweeds was purified by column chromatography and each fraction was eluted by using petroleum ether and ethyl acetate as solvent combination. The analysis of the purified fraction of H. musciformis and K. alvarezii revealed the presence of 8 terpenoid fractions, and G. dura showed only 4 major components respectively. Vero cell lines were employed in the antiviral assays, infected to CHIKV, and treated with varied doses of purified terpenoid extracts. In the antiviral activity, terpenoid extracts of G. dura showed remarkable and promising EC50 inhibitory effect at 1.25 μg/ml. Further, the terpenoid extracts displayed efficient virucidal activity against CHIKV (inhibit around 90%) with 5 μg/ml dosage. As the last phase, terpenoid extracts added at time intervals of 0, 1, 2, 3 post-infection periods still maintained a significant inhibitory potential against CHIKV viral replication. Thus, the overall study suggests that the terpenoid extracts of G. dura may be effectively used in the prevention and treatment of CHIKV infections. Clinical studies may be warranted for designing a promising new anti-CHIKV drug.en_US
dc.identifier.affiliationsSree Neelakanta Government Sanskrit College Pattambi, Palakkad-679306, Kerala, Indiaen_US
dc.identifier.affiliationsChristian College, Kattakada- 695572, Kerala, Indiaen_US
dc.identifier.affiliationsCenter for Innovation in Science and Social Action (CISSA), Thiruvananthapuram-695010, Kerala, Indiaen_US
dc.identifier.citationSumayya S. S., Lubaina A. S., Murugan K.. Screening Of In Vitro Antiviral Activity Of Purified Terpenoid Extracts Of Selected Sea Weeds Against Chikungunya Virus. International Journal of Pharmaceutical Sciences and Drug Research. 2020 Jul; 12(4): 320-324en_US
dc.identifier.issn0975-248X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/206327
dc.languageenen_US
dc.publisherMRI Publication Pvt. Ltd.en_US
dc.relation.issuenumber4en_US
dc.relation.volume12en_US
dc.source.urihttps://doi.org/10.25004/IJPSDR.2020.120403en_US
dc.subjectChikungunya virusen_US
dc.subjectsea weedsen_US
dc.subjectantiviralen_US
dc.subjectterpenoid extractsen_US
dc.subjectpurificationen_US
dc.titleScreening Of In Vitro Antiviral Activity Of Purified Terpenoid Extracts Of Selected Sea Weeds Against Chikungunya Virusen_US
dc.typeJournal Articleen_US
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