Comparative Evaluation of GENE-XPERT MTB/RIF with AFB Smear Microscopy Methods for the Diagnosis of Tuberculosis in Sputum Specimens of Suspected Pulmonary Tuberculosis Patients

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dc.contributor.authorKhan, Rehbaren_US
dc.contributor.authorAhmad, Men_US
dc.contributor.authorRaghav, Santosh Kumaren_US
dc.contributor.authorRastogi, Swarnaen_US
dc.date.accessioned2020-01-02T06:08:25Z
dc.date.available2020-01-02T06:08:25Z
dc.date.issued2019-07
dc.description.abstractTuberculosis is a major public health problem worldwide and remains one of the most significant causes of death from an infectious agent. India contributes to 25.5% of the global new TB case detection. In recent times, emphasis has shifted from older phenotypically and biochemical methods of diagnosis to molecular methods such as GeneXpert MTB/RIF, a real time PCR that can detect MTB and rifampicin resistence simultaneously. Methods: This study aims at assessing the performance of acid fast bacilli (AFB) smear microscopy in comparison with GeneXpert MTB RIF, in the diagnosis of pulmonary tuberculosis. Study was a cross-sectional study carried out at the Department of Pulmonary Medicine, Muzaffarnagar Medical College, from January 2018 to April 2019. Result: The detection of MTB and rifampicin resistance using the Xpert MTB/RIF assay was assessed in 67 specimens from patients suspected of having pulmonary tuberculosis and compared with conventional smear microscopy. Out of these 67 sputum specimens, 38 samples were MTB positive by smear microscopy while 56 samples were MTB detected by Gene Xpert assay. Gene Xpert detected 18 additional tubercular cases and identify two cases of Rifampicin resistant MTB. Conclusion: Study show that there was no statistically significance in diagnostic value between GeneXpert and AFB smear microscopy in sputum samples however Gene Xpert MTB/RIF is useful method for rapid detection of MTB and Rifampicin resistance simultaneously.en_US
dc.identifier.affiliationsDepartment of Pulmonary Medicine, Muzaffarnagar Medical College, Muzaffarnagar.en_US
dc.identifier.affiliationsDepartment of Community Medicine, Muzaffarnagar Medical College, Muzaffarnagaren_US
dc.identifier.citationKhan Rehbar, Ahmad M, Raghav Santosh Kumar, Rastogi Swarna. Comparative Evaluation of GENE-XPERT MTB/RIF with AFB Smear Microscopy Methods for the Diagnosis of Tuberculosis in Sputum Specimens of Suspected Pulmonary Tuberculosis Patients. Annals of International medical and Dental Research. 2019 Jul; 5(4): 10-13en_US
dc.identifier.issn2395-2822
dc.identifier.issn2395-2814
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/188917
dc.languageenen_US
dc.publisherSociety for Health Care & Research Developmenten_US
dc.relation.issuenumber4en_US
dc.relation.volume5en_US
dc.source.urihttps://doi.org/10.21276/aimdr.2019.5.4.TB4en_US
dc.subjectDirect smear microscopyen_US
dc.subjectGeneXpert MTB/RIF assayen_US
dc.subjectpulmonary tuberculosisen_US
dc.titleComparative Evaluation of GENE-XPERT MTB/RIF with AFB Smear Microscopy Methods for the Diagnosis of Tuberculosis in Sputum Specimens of Suspected Pulmonary Tuberculosis Patientsen_US
dc.typeJournal Articleen_US
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