Purification and characterization of a Ca(2+)-dependent/calmodulin-stimulated protein kinase from moss chloronema cells.

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dc.contributor.authorD'Souza, Jacinta Sen_US
dc.contributor.authorJohri, Man Mohanen_US
dc.date.accessioned2003-03-25en_US
dc.date.accessioned2009-06-01T14:30:23Z
dc.date.available2003-03-25en_US
dc.date.available2009-06-01T14:30:23Z
dc.date.issued2003-03-25en_US
dc.description.abstractWe have demonstrated the presence of a Ca2+-dependent/calmodulin-stimulated protein kinase (PK) in chloronema cells of the moss Funaria hygrometrica. The kinase, with a molecular mass of 70,000 daltons (PK70), was purified to homogeneity using ammonium sulphate fractionation, DEAE-cellulose chromatography, and calmodulin (CaM)-agarose affinity chromatography. The kinase activity was stimulated at a concentration of 50 mM free Ca2+, and was further enhanced 3-5-fold with exogenously added 3-1000 nm moss calmodulin (CaM). Autophosphorylation was also stimulated with Ca2+ and CaM. Under in vitro conditions, PK70 phosphorylated preferentially lysine-rich substrates such as HIIIS and HVS. This PK shares epitopes with the maize Ca2+-dependent/calmodulin-stimulated PK (CCaMK) and also exhibits biochemical properties similar to the maize, lily, and tobacco CCaMK. We have characterized it as a moss CCaMK.en_US
dc.description.affiliationDepartment of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.en_US
dc.identifier.citationD'Souza JS, Johri MM. Purification and characterization of a Ca(2+)-dependent/calmodulin-stimulated protein kinase from moss chloronema cells. Journal of Biosciences. 2003 Mar; 28(2): 223-33en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110875
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshBryopsida --cytologyen_US
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinase Type 2en_US
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases --isolation & purificationen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.titlePurification and characterization of a Ca(2+)-dependent/calmodulin-stimulated protein kinase from moss chloronema cells.en_US
dc.typeJournal Articleen_US
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