Minimum inhibitory concentration of drugs against Mycobacterium leprae as determined by an in vitro assay.
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Date
1986-03
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Abstract
The observations that live Mycobacterium leprae after entry into cultured
peritoneal macrophages from mice, reduced the EA rosetting macrophages, have been
exploited to determine the minimum inhibitory concentration of diamino diphenyl sulphone
and rifampicin. Diamino diphenyl sulphone showed a minimum inhibitory concentration of
0·028 μg/ml and rifampicin 0·11 μg/ml when given externally. However, there was accumulation
of diamino diphenyl sulphone inside the macrophages. At an external concentration of
0·028 μg/ml the concentration inside the macrophage was 0·5 μg/ml. The minimum inhibitory
concentration for diamino diphenyl sulphone in this assay system is higher by several folds and
that for rifampicin is slightly lower, than what is reported earlier with mice foot pad
experiments. The minimum inhibitory concentration reported in this assay system is quite
close to what is observed for in vitro inhibition of Mycobacterium lufu with both the drugs.
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Keywords
Mycobacterium leprae, minimum inhibition concentration, drugs, Fc receptor assay
Citation
Jagannathan R, Mahadevan P R. Minimum inhibitory concentration of drugs against Mycobacterium leprae as determined by an in vitro assay. Journal of Biosciences. 1986 Mar; 10(1): 137-144.