B cells are the principal circulating mononuclear cells infected by dengue virus.

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dc.contributor.authorKing, A Den_US
dc.contributor.authorNisalak, Aen_US
dc.contributor.authorKalayanrooj, Sen_US
dc.contributor.authorMyint, K Sen_US
dc.contributor.authorPattanapanyasat, Ken_US
dc.contributor.authorNimmannitya, Sen_US
dc.contributor.authorInnis, B Len_US
dc.date.accessioned2009-05-27T16:36:59Z
dc.date.available2009-05-27T16:36:59Z
dc.date.issued1999-12-06en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractAlthough dengue virus infects a variety of cells in vitro, little is known about cell types infected in vivo. Since blood is a readily accessible tissue, we chose to determine which circulating blood cells are infected by dengue viruses. We collected blood mononuclear cells from acutely ill dengue patients and separated the cells by flow cytometry into subsets for virus isolation. Cells were sorted into groups corresponding to the cluster designations CD3, CD14, CD16 and CD20. Virus was isolated from sorted groups by inoculation into Toxorhynchites splendens mosquitos. The majority of the virus was recovered from the CD20 or B cell positive subset. Little virus was isolated from monocytes, NK cells or T cells. Virus was isolated from B cells regardless of the age or sex of the patient, virus serotype isolated, or the patient's history of dengue virus infection. The location of cell associated virus was determined by proteolytic digestion of surface virus. There was an equal distribution of virus between the intracellular compartment and the surface of B cells. The intracellular localization of virus was confirmed by immunocytochemistry. Since this study focused on circulating cells, no inferences were made regarding infection of cells in solid tissues.en_US
dc.description.affiliationArmed Forces Research Institute of Medical Sciences, Bangkok, Thailand.. alan@cytopulse.comen_US
dc.identifier.citationKing AD, Nisalak A, Kalayanrooj S, Myint KS, Pattanapanyasat K, Nimmannitya S, Innis BL. B cells are the principal circulating mononuclear cells infected by dengue virus. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Dec; 30(4): 718-28en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35621
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/1999-30-4/1999-30-4-718.pdfen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal --diagnostic useen_US
dc.subject.meshB-Lymphocyte Subsets --virologyen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshCulicidaeen_US
dc.subject.meshDengue --immunologyen_US
dc.subject.meshDengue Virus --immunologyen_US
dc.subject.meshExtracellular Matrix --virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIntracellular Membranes --virologyen_US
dc.subject.meshMaleen_US
dc.subject.meshVirus Cultivationen_US
dc.titleB cells are the principal circulating mononuclear cells infected by dengue virus.en_US
dc.typeJournal Articleen_US
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