Harmaline interactions with yeast invertase.

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dc.contributor.authorGill, R Ken_US
dc.contributor.authorKaur, Jen_US
dc.contributor.authorMahmood, Sen_US
dc.contributor.authorNagpaul, J Pen_US
dc.contributor.authorMahmood, Aen_US
dc.date.accessioned1998-04-01en_US
dc.date.accessioned2009-05-27T09:19:02Z
dc.date.available1998-04-01en_US
dc.date.available2009-05-27T09:19:02Z
dc.date.issued1998-04-01en_US
dc.description.abstractThe effect of harmaline, a plant alkaloid has been studied on yeast invertase activity in the absence and presence of 50mM Na+ as a function of pH. Harmaline (1-3 mM) inhibited the invertase activity at pH 5.2, 6.8 and 8 both in the absence (44-92%) and (22-85%) of Na+ ions. Kinetic analysis revealed that harmaline is a non-competitive inhibitor of invertase, at pH 5.2 and 6.8 but at pH 8, it produced a mixed type of inhibition, Km increased by 450% and 175% and Vmax decreased by 82% and 63% in the absence and presence of 50mM Na ions respectively. The observed inhibition of invertase by harmaline was reversible in nature. These findings suggest that the presence of Na+ site is not a prerequisite for the inhibition of enzyme by harmaline.en_US
dc.description.affiliationDepartment of Biochemistry, Panjab University, Chandigarh.en_US
dc.identifier.citationGill RK, Kaur J, Mahmood S, Nagpaul JP, Mahmood A. Harmaline interactions with yeast invertase. Indian Journal of Biochemistry & Biophysics. 1998 Apr; 35(2): 86-90en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27134
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshGlycoside Hydrolases --chemistryen_US
dc.subject.meshHarmaline --pharmacologyen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshKineticsen_US
dc.subject.meshSaccharomyces cerevisiae --enzymologyen_US
dc.subject.meshbeta-Fructofuranosidaseen_US
dc.titleHarmaline interactions with yeast invertase.en_US
dc.typeJournal Articleen_US
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