Comparative analysis of duodenal biopsy tissue orientation supported on vegetable matrix versus filter paper.

dc.contributor.authorNada, Ritambhraen_US
dc.contributor.authorDuseja, Ajayen_US
dc.contributor.authorSachdev, Atulen_US
dc.contributor.authorMohan, Harshen_US
dc.date.accessioned2002-05-18en_US
dc.date.accessioned2009-05-29T02:08:56Z
dc.date.available2002-05-18en_US
dc.date.available2009-05-29T02:08:56Z
dc.date.issued2002-05-18en_US
dc.description.abstractBACKGROUND: Biopsy tissue may get disoriented if filter paper is used as a supporting medium. Use of vegetable matrix (cucumber) as a supporting medium may obviate the need of lifting the tissue while making blocks and thus avoid disorientation that occurs during this step. OBJECTIVE: To compare the orientation of duodenal biopsy tissue supported on vegetable matrix (cucumber) and on filter paper. METHODS: Over one year, 40 patients (20 with large-volume diarrhea, 20 with dyspepsia) were included in the study. Two pairs of duodenal biopsy tissues were obtained during gastroscopy; one pair was placed on filter paper, the other on vegetable matrix. Tissue and vegetable matrix were embedded together while making blocks, whereas the tissue had to be lifted off in case of filter paper. Sections were stained and assessed for crypt-villous alignment, parallel orientation of crypts and presence of visible muscularis mucosae with the help of a scoring system. RESULTS: Compared to biopsy tissue supported on filter paper, vegetable matrix-supported tissues were better oriented. Scores were rated as bad, good and very good in 8, 11 and 21 vegetable matrix-mounted tissues and in 21, 11 and 8 filter paper-mounted tissues, respectively. CONCLUSION: Duodenal biopsy tissue supported on vegetable matrix (cucumber) is better oriented than that on filter paper.en_US
dc.description.affiliationDepartment of Pathology, Government Medical College and Hospital, Chandigarh.en_US
dc.identifier.citationNada R, Duseja A, Sachdev A, Mohan H. Comparative analysis of duodenal biopsy tissue orientation supported on vegetable matrix versus filter paper. Indian Journal of Gastroenterology. 2002 May-Jun; 21(3): 110-2en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/63793
dc.language.isoengen_US
dc.source.urihttps://www.indianjgastro.comen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshChilden_US
dc.subject.meshChronic Diseaseen_US
dc.subject.meshCucumis sativusen_US
dc.subject.meshDiarrhea --pathologyen_US
dc.subject.meshDuodenum --pathologyen_US
dc.subject.meshDyspepsia --pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshTissue Embedding --instrumentationen_US
dc.titleComparative analysis of duodenal biopsy tissue orientation supported on vegetable matrix versus filter paper.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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