2-Deoxy-D-glucose induced modulation of DNA damage repair, survival, mutagenesis and recombinogenesis in 8-MOP+UVA treated yeast.

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dc.contributor.authorBala, Men_US
dc.contributor.authorJain, Ven_US
dc.date.accessioned1997-12-22en_US
dc.date.accessioned2009-05-27T09:58:48Z
dc.date.available1997-12-22en_US
dc.date.available2009-05-27T09:58:48Z
dc.date.issued1997-12-22en_US
dc.description.abstractCellular and genomic effects of post-treatment repair modulation by 2-deoxy-D-glucose (2-DG) and yeast extract were studied in 8-MOP + UVA treated cells of Saccharomyces cerevisiae. The type of lesions and their repair in phosphate buffer glucose (PBG) differed with UVA dose. At low UVA dose (1.4 kJ/m2), lesions were sublethal and mutagenic and did not repair by recombinogensis. The fraction of potentially lethal lesions and lesions repaired by recombinogenesis increased with UVA dose. Cellular repair in PBG was largely error-free and was inhibited by 2-DG. Yeast extract enhanced cellular repair and also recombinogensis; 2-DG in presence of yeast extract promoted error-prone repair. Pulsed-field gel electrophoresed chromosomal DNA bands did not show observable alterations immediately after 8-MOP + UVA treatment. On post-treatment incubation in PBG, the intensity ratio (rho n), of each band altered in a biphasic manner showing decrease first, followed by either increase or no change upto 24 hr depending upon UVA exposure dose. Presence of 2-DG in PBG inhibited decrease in rho n in a concentration dependent manner. Yeast extract reduced the time of first phase of DNA repair. 2-DG and yeast extract together reduced the time of first phase of repair and also inhibited the subsequent increase in rho n, which was observed in the case of yeast extract in PBG. It is proposed that (i) 2-DG in PBG inhibits excision of DNA damage and error-free repair; (ii) yeast extract stimulates the error-prone repair associated with cell cycle and recombinogenesis; (iii) 2-DG in presence of yeast extract allows excision of damage but inhibits build up through recombinogenesis inducing instead, cell cycle associated error-prone repair. A simple schematic model has been proposed to explain these events.en_US
dc.description.affiliationDepartment of Biocybernetics, Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi, India.en_US
dc.identifier.citationBala M, Jain V. 2-Deoxy-D-glucose induced modulation of DNA damage repair, survival, mutagenesis and recombinogenesis in 8-MOP+UVA treated yeast. Indian Journal of Biochemistry & Biophysics. 1997 Dec; 34(6): 483-93en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/29125
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshDNA Damageen_US
dc.subject.meshDNA Repair --drug effectsen_US
dc.subject.meshDNA, Fungal --drug effectsen_US
dc.subject.meshGene Conversion --drug effectsen_US
dc.subject.meshMethoxsalen --pharmacologyen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshMutagenesisen_US
dc.subject.meshRecombination, Genetic --drug effectsen_US
dc.subject.meshSaccharomyces cerevisiae --drug effectsen_US
dc.subject.meshUltraviolet Raysen_US
dc.title2-Deoxy-D-glucose induced modulation of DNA damage repair, survival, mutagenesis and recombinogenesis in 8-MOP+UVA treated yeast.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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