Novel multi-layer APPPA microcapsules for oral delivery: preparation condition, stability and permeability.

Simple item page
dc.contributor.authorOuyang, Wei
dc.contributor.authorChen, Hongmei
dc.contributor.authorJones, Mitchell L
dc.contributor.authorHaque, Tasima
dc.contributor.authorMartoni, Christopher
dc.contributor.authorAfkhami, Fateme
dc.contributor.authorPrakash, Satya
dc.date.accessioned2011-11-11T08:38:33Z
dc.date.available2011-11-11T08:38:33Z
dc.date.issued2009-12
dc.description.abstractOral therapy utilizing cell microencapsulation has shown promise in the treatment of many diseases. Current obtainable microcapsule membranes, however, show inadequate stability in the gastrointestinal (GI) environment, thus restricting the general application of live cells for oral therapy. To overcome this limitation, we have previously developed a novel multi-layer alginate/poly-L-lysine/pectin/poly-L-lysine/alginate microcapsule (APPPA) with demonstrated improvement on membrane stability over the frequently reported alginate/poly-L-lysine/alginate (APA) microcapsules. In this study, we further examined the effects of preparation conditions on microcapsule formation, and assessed the membrane strength and GI stability. Results showed that increased membrane strength of the APPPA microcapsules was attained by using pectin with low degree of esterification as the mid-layer material, saline as the solvent for the preparation solutions and washing medium, and 0.1 M CaCl2 as the gelling solution for alginate cores. Resistance of this membrane to the simulated GI fluids was also investigated. Permeability of and release profiles from the APPPA microcapsules were found comparable to the APA microcapsules. These findings suggested that the multi-layer APPPA microcapsule formulation may have potential in oral delivery of proteins, live bacterial cells and other biomedical applications.en_US
dc.identifier.citationOuyang Wei, Chen Hongmei, Jones Mitchell L, Haque Tasima, Martoni Christopher, Afkhami Fateme, Prakash Satya. Novel multi-layer APPPA microcapsules for oral delivery: preparation condition, stability and permeability. Indian Journal of Biochemistry & Biophysics. 2009 Dec; 46(6): 491-497.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135232
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/7252/1/IJBB%2046%286%29%20491-497.pdfen_US
dc.subjectMicrocapsuleen_US
dc.subjectAPPPAen_US
dc.subjectMembrane Strengthen_US
dc.subjectGI stabilityen_US
dc.subjectArtificial Cellsen_US
dc.subjectOral Deliveryen_US
dc.subjectPectinen_US
dc.subject.meshAdministration, Oral
dc.subject.meshAlginates --administration & dosage
dc.subject.meshAlginates --chemistry
dc.subject.meshAlginates --metabolism
dc.subject.meshAnimals
dc.subject.meshCalcium Chloride --chemistry
dc.subject.meshCapsules
dc.subject.meshCattle
dc.subject.meshCell Membrane Permeability
dc.subject.meshDrug Compounding --methods
dc.subject.meshDrug Stability
dc.subject.meshGastrointestinal Tract --metabolism
dc.subject.meshPectins --chemistry
dc.subject.meshSodium --chemistry
dc.subject.meshSodium Chloride --chemistry
dc.titleNovel multi-layer APPPA microcapsules for oral delivery: preparation condition, stability and permeability.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijbb2009v46n6p491.pdf
Size:
2.03 MB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics