An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine.

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dc.contributor.authorSingh, Aen_US
dc.contributor.authorKulshreshtha, Ren_US
dc.contributor.authorMathur, Aen_US
dc.date.accessioned2000-03-29en_US
dc.date.accessioned2009-06-01T14:53:20Z
dc.date.available2000-03-29en_US
dc.date.available2009-06-01T14:53:20Z
dc.date.issued2000-03-29en_US
dc.description.abstractJapanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92 2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.en_US
dc.description.affiliationPostgraduate Department of Microbiology, KG's Medical College, Lucknow 226 003, India.en_US
dc.identifier.citationSingh A, Kulshreshtha R, Mathur A. An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine. Journal of Biosciences. 2000 Mar; 25(1): 47-55en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111150
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChemokine CCL22en_US
dc.subject.meshChemokines, CC --analysisen_US
dc.subject.meshEncephalitis Virus, Japaneseen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshMacrophages --metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleAn enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine.en_US
dc.typeJournal Articleen_US
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