Development of the rapid and simplified ELISA (WHOLE BLOOD-ELISA) using samples of Schistosoma japonicum-infected human whole blood.

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dc.contributor.authorHirose, Yoshinorien_US
dc.contributor.authorKirinoki, Masashien_US
dc.contributor.authorLipayon, Ignacio Len_US
dc.contributor.authorBlas, Bayani Len_US
dc.contributor.authorChigusa, Yuichien_US
dc.contributor.authorMatsuda, Hajimeen_US
dc.date.accessioned2009-05-27T15:44:47Z
dc.date.available2009-05-27T15:44:47Z
dc.date.issued2005-01-24en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractAn ELISA technique was developed using samples of Schistosoma japonicum-infected human whole blood based on the conventional ELISA. In this study, the following were demonstrated. 1) Whole blood samples could be used. 2) The volume of whole blood and conjugate could be reduced to 0.05 ml. 3) The incubation time was shortened to 5 minutes. 4) The optical density could be measured at 10 minutes after transferring the substrate and the volume was reduced to 0.1 ml. 5) It did not require a fixed temperature setting. 6) The operation time was as short as 20 to 30 minutes. 7) The optical density values were almost the same as the conventional ELISA and were not influenced by other common intestinal helminthic infections. 8) The observed variations from day to day including effects of sampling in stool examination were negated by the results of this ELISA technique. 9) Based on correlation with stool examination results, criteria can be formulated in which optical density values of 0.3 and above as positive, 0.1 to less than 0.3 as doubtful, and less than 0.1 as negative. Whenever an immunological field survey is necessary, before and after a selective or a mass treatment control program, this WHOLE BLOOD-ELISA, which was shown to be rapid and simple, is recommended.en_US
dc.description.affiliationDepartment of Tropical Medicine and Parasitology, Dokkyo University School of Medicine, Mibu, Shimotsuga, Tochigi, Japan.en_US
dc.identifier.citationHirose Y, Kirinoki M, Lipayon IL, Blas BL, Chigusa Y, Matsuda H. Development of the rapid and simplified ELISA (WHOLE BLOOD-ELISA) using samples of Schistosoma japonicum-infected human whole blood. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 Jan; 36(1): 72-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/33559
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2005_36_1/10-3354.pdfen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Helminth --blooden_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMass Screeningen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshSchistosoma japonicum --immunologyen_US
dc.subject.meshSchistosomiasis --blooden_US
dc.titleDevelopment of the rapid and simplified ELISA (WHOLE BLOOD-ELISA) using samples of Schistosoma japonicum-infected human whole blood.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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