Expression of Mycobacterium tuberculosis genes in Escherichia coli.
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Date
1986-06
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Abstract
Two Escherichia coli clones expressing Mycobacterium tuberculosis antigens were
isolated from a gene-bank in the plasmid vector pBR 325. 'Western blot' analysis revealed the
presence of a unique protein band of molecular weight 68,000 and 38,000, respectively in cellextracts
from each clone. The 68,000 dalton antigen was found to be expressed on Escherichia
coli outer surface. Plasmid DNA from a third clone could confer leucine independence on two
different leu Β mutants of Escherichia coli but not on mutants in other leu genes, pointing to
the possibility ofgenetic complementation. Thus, Mycobacterium tuberculosis DNA is capable
of expression in Escherichia coli.
Description
Keywords
Mycobacterium tuberculosis gene-expression, western blot, cloned M. tuberculosis, DNA
Citation
Bhattacharya Subha, Ranadive Satish N, Bhattacharya Alok. Expression of Mycobacterium tuberculosis genes in Escherichia coli. Journal of Biosciences. 1986 June; 10(2): 277-281.