Analysis of connective tissue growth factor promoter polymorphism in Thai children with biliary atresia.

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dc.contributor.authorSa-nguanmoo, Pattaratidaen_US
dc.contributor.authorVejchapipat, Paisarnen_US
dc.contributor.authorChongsrisawat, Voranushen_US
dc.contributor.authorChirathaworn, Chintanaen_US
dc.contributor.authorHonsawek, Sittisaken_US
dc.contributor.authorTheamboonlers, Apiradeeen_US
dc.contributor.authorPoovorawan, Yongen_US
dc.date.accessioned2009-05-27T18:37:00Z
dc.date.available2009-05-27T18:37:00Z
dc.date.issued2007-02-23en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractOBJECTIVE: Connective tissue growth factor (CTGF) has been proposed to play a key role in the pathogenesis of hepatic fibrosis in biliary atresia (BA). The aim of the present study was to determine the single nucleotide polymorphism (SNP) in the promoter region of CTGF gene in a Thai population, and to investigate the possible role of CTGF promoter polymorphism in the susceptibility of BA. MATERIAL AND METHOD: Genomic DNA was obtained from 84 patients with BA and 142 healthy controls. The -447 G/C and -132 C/G in CTGF promoter were amplified and examined by amplification-refractory mutation system (ARMs) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, respectively. The test of Hardy-Weinberg equilibrium (HWE) was performed using HWE program of SNPAnalyzer. Statistical analysis was carried out with SPSS and Epi Info. RESULT: According to the previous experiment, there were two SNPs, which were at position -447 and -132 on the promoter. However, there was only one SNP at the position -447 in the Thai population. No significant differences in genotype and allele frequency were observed between BA and controls or with BA subgroups. CONCLUSION: The present study demonstrated that CTGF polymorphism at -447 G/C was not associated with BA and the jaundice status of the postoperative BA patients.en_US
dc.description.affiliationCenter of Excellence in Viral Hepatitis Research, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University.en_US
dc.identifier.citationSa-nguanmoo P, Vejchapipat P, Chongsrisawat V, Chirathaworn C, Honsawek S, Theamboonlers A, Poovorawan Y. Analysis of connective tissue growth factor promoter polymorphism in Thai children with biliary atresia. Journal of the Medical Association of Thailand. 2007 Feb; 90(2): 251-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/39507
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAllelesen_US
dc.subject.meshBiliary Atresia --geneticsen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshChilden_US
dc.subject.meshConnective Tissueen_US
dc.subject.meshConnective Tissue Growth Factoren_US
dc.subject.meshDisease Susceptibilityen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHumansen_US
dc.subject.meshImmediate-Early Proteins --geneticsen_US
dc.subject.meshIntercellular Signaling Peptides and Proteins --geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshPolymorphism, Single Nucleotideen_US
dc.subject.meshRisk Factorsen_US
dc.subject.meshThailanden_US
dc.titleAnalysis of connective tissue growth factor promoter polymorphism in Thai children with biliary atresia.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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