Effects of keratinocyte-derived and fibroblast-derived exosomes on human epidermal melanocytes

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dc.contributor.authorShi, Hai-Xiaen_US
dc.contributor.authorZhang, Ru-Zhien_US
dc.contributor.authorXiao, Lien_US
dc.contributor.authorWang Lien_US
dc.date.accessioned2023-08-10T07:29:57Z
dc.date.available2023-08-10T07:29:57Z
dc.date.issued2022-06
dc.description.abstractBackground: Exosomes have been demonstrated to carry proteins, membrane lipids, mRNAs and microRNAs which can be transferred to surrounding cells and regulate the functions of those recipient cells. Objectives: The objective of the study was to investigate the effects of exosomes released by keratinocytes and fibroblasts on the proliferation, tyrosinase activity and melanogenesis of melanocytes. Methods: Melanocytes, keratinocytes and fibroblasts obtained from human foreskin were cultured and exosomes secreted by keratinocytes and fibroblasts were harvested from the culture supernatants by ultracentrifugation. Each exosome fraction was divided into two parts; one part was subjected to high-throughput sequencing using an Illumina HiSeq sequencer to characterize the microRNA expression profiles, while the other part was labeled with the fluorescent dye PKH67 and was then co-cultivated with epidermal melanocytes. Results: High-throughput sequencing analysis showed 168 differentially expressed microRNA within exosomes derived from keratinocytes and from fibroblasts, 97 of those being up-regulated with the other 71 down-regulated. Gene ontology analysis showed that the target genes responsible for these differentially expressed microRNAs were mainly enriched in the protein-binding region of molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that target genes regulated by differentially expressed microRNA were mainly involved in mitogen-activated protein kinase (MAPK) signaling pathway, Ras signaling pathway, cAMP signaling pathway and Wnt signaling pathway. Keratinocyte-derived exosomes were taken up by melanocytes co-cultured with them and promoted the proliferation, tyrosinase activity and melanin synthesis of those melanocytes. However, fibroblast-derived exosomes had no similar effects on melanocytes. Conclusion: Keratinocyte-derived exosomes but not fibroblast-derived exosomes were taken up by melanocytes in co-culture and significantly stimulated their proliferation, tyrosinase activity and melanin synthesis. Those different effects may be mainly due to the differential expression of microRNAs in exosomes derived from the different types of cells. Limitations: Electron microscopy of the obtained exosomes and in-depth study of apparently differentially expressed microRNAs were not performeden_US
dc.identifier.affiliationsDepartment of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhouen_US
dc.identifier.affiliationsDepartment of Dermatology, The First Affiliated Hospital of Bengbu Medical College, Anhui, Chinaen_US
dc.identifier.citationShi, Hai-Xiaen_US
dc.identifier.citationZhang, Ru-Zhien_US
dc.identifier.citationXiao, Lien_US
dc.identifier.citationWang Li. Effects of keratinocyte-derived and fibroblast-derived exosomes on human epidermal melanocytes. Indian Journal of Dermatology, Venereology and Leprology. 2022 Jun; 88(3): 322-331en_US
dc.identifier.issn0973-3922
dc.identifier.issn0378-6323
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223010
dc.languageenen_US
dc.publisherScientific Scholaren_US
dc.relation.issuenumber3en_US
dc.relation.volume88en_US
dc.source.urihttps://doi.org/10.25259/IJDVL_1087_19en_US
dc.subjectExosomesen_US
dc.subjectfibroblastsen_US
dc.subjecthigh-throughput sequencingen_US
dc.subjectkeratinocytesen_US
dc.subjectmelanocytesen_US
dc.subjectmicroRNAsen_US
dc.titleEffects of keratinocyte-derived and fibroblast-derived exosomes on human epidermal melanocytesen_US
dc.typeJournal Articleen_US
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