Infectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna in Vigna mungo and Vigna radiata.

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dc.contributor.authorBalaji, Ven_US
dc.contributor.authorVanitharani, Ren_US
dc.contributor.authorKarthikeyan, A Sen_US
dc.contributor.authorAnbalagan, Sen_US
dc.contributor.authorVeluthambi, Ken_US
dc.date.accessioned2004-09-24en_US
dc.date.accessioned2009-06-01T15:10:21Z
dc.date.available2004-09-24en_US
dc.date.available2009-06-01T15:10:21Z
dc.date.issued2004-09-24en_US
dc.description.abstractMungbean yellow mosaic virus-Vigna (MYMV-Vig), a Begomovirus that causes yellow mosaic disease, was cloned from field-infected blackgram (Vigna mungo). One DNA A clone (KA30) and five different DNA B clones (KA21, KA22, KA27, KA28 and KA34) were obtained. The sequence identity in the 150-nt common region (CR) between DNA A and DNA B was highest (95%) for KA22 DNA B and lowest (85.6%) for KA27 DNA B. The Rep-binding domain had three complete 11-nt (5'-TGTATCGGTGT-3') iterons in KA22 DNA B (and KA21, KA28 and KA34), while the first iteron in KA27 DNA B (5'-ATCGGTGT-3') had a 3-nt deletion. KA27 DNA B, which exhibited 93.9% CR sequence identity to the mungbean-infecting MYMV, also shared the 3-nt deletion in the first iteron besides having an 18-nt insertion between the third iteron and the conserved nonanucleotide. MYMV was found to be closely related to KA27 DNA B in amino acid sequence identity of BV1 (94.1%) and BC1 (97.6%) proteins and in the organization of nuclear localization signal (NLS), nuclear export signal (NES) and phosphorylation sites. Agroinoculation of blackgram (V. mungo) and mungbean (V. radiata) with partial dimers of KA27 and KA22 DNA Bs along with DNA A caused distinctly different symptoms. KA22 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in blackgram. In contrast, KA27 DNA B caused more intense yellow mosaic symptoms with high viral DNA titre in mungbean. Thus, DNA B of MYMVVig is an important determinant of host-range between V. mungo and V. radiata.en_US
dc.description.affiliationCentre for Plant Molecular Biology, School of Biotechnology, Madurai Kamaraj University, 625 021, India.en_US
dc.identifier.citationBalaji V, Vanitharani R, Karthikeyan AS, Anbalagan S, Veluthambi K. Infectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna in Vigna mungo and Vigna radiata. Journal of Biosciences. 2004 Sep; 29(3): 297-308en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111353
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAmino Acid Motifsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshDNA, Viral --analysisen_US
dc.subject.meshGeminiviridae --geneticsen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshPhaseolus --classificationen_US
dc.subject.meshPlant Diseases --virologyen_US
dc.subject.meshPlant Leaves --virologyen_US
dc.subject.meshPlasmids --metabolismen_US
dc.subject.meshSeeds --virologyen_US
dc.subject.meshSequence Analysis, DNAen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshVirulenceen_US
dc.titleInfectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna in Vigna mungo and Vigna radiata.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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