Detection and differentiation of human herpesviruses 1-5 by consensus primer PCR and RFLP.

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dc.contributor.authorSakulwira, Kamolen_US
dc.contributor.authorVanapongtipagorn, Pijitraen_US
dc.contributor.authorTheamboonlers, Apiradeeen_US
dc.contributor.authorBhattarakosol, Parvapanen_US
dc.contributor.authorWananukul, Siriwanen_US
dc.contributor.authorPoovorawan, Yongen_US
dc.date.accessioned2009-05-27T17:15:35Z
dc.date.available2009-05-27T17:15:35Z
dc.date.issued2003-03-23en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractEight human viruses of the Herpesviridae family represent a significant public health problem world-wide. Detection and typing of five of the human herpesviruses (HSV-1, HSV-2, VZV, EBV, and CMV) was performed by applying a consensus primer polymerase chain reaction (PCR). The amplified PCR products from the five human herpesviruses were typed based on their restriction enzyme digestion polymorphism with Hinf I and Alu I. Fifteen clinically suspected specimens from herpesvirus-infected patients were also evaluated. A fragment of the DNA polymerase gene from each of the five human herpesviruses was successfully amplified by the set of consensus primers. Their amplicons obtained by PCR from the template DNAs were subjected to restriction endonuclease digestion and human herpesviruses 1-5 could be clearly differentiated and typed. This method can be used to detect and differentiate between the five human herpesviruses in clinical specimens. This study demonstrates the value of testing for five human herpesviruses by consensus PCR and restricted fragment length polymorphism (RFLP). These procedures are simple and straightforward techniques for the investigation of clinical specimens.en_US
dc.description.affiliationInter-Department of Medical Microbiology, Faculty of Graduate Studies, Chulalongkorn University, Bangkok 10330, Thailand.en_US
dc.identifier.citationSakulwira K, Vanapongtipagorn P, Theamboonlers A, Bhattarakosol P, Wananukul S, Poovorawan Y. Detection and differentiation of human herpesviruses 1-5 by consensus primer PCR and RFLP. Asian Pacific Journal of Allergy and Immunology. 2003 Mar; 21(1): 55-61en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36800
dc.language.isoengen_US
dc.subject.meshBase Sequence --geneticsen_US
dc.subject.meshChilden_US
dc.subject.meshDNA, Viral --geneticsen_US
dc.subject.meshHerpesviridae --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.titleDetection and differentiation of human herpesviruses 1-5 by consensus primer PCR and RFLP.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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