Identification of various Candida species isolated from patients with vulvovaginal candidiasis attending a tertiary care hospital in North India

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dc.contributor.authorJahan, Nooren_US
dc.contributor.authorVishnoi, Pratishthaen_US
dc.contributor.authorGupta, Bhavanaen_US
dc.contributor.authorSrivastava, Swatien_US
dc.contributor.authorAhmad, Sirajen_US
dc.contributor.authorKhatoon, Raziaen_US
dc.date.accessioned2020-04-09T07:43:29Z
dc.date.available2020-04-09T07:43:29Z
dc.date.issued2020-02
dc.description.abstractBackground: Vulvovaginal Candidiasis (VVC) is a condition diagnosed in a large proportion of women presenting with complaints of abnormal vaginal discharge. The risk of VVC is high in women with diabetes mellitus, pregnancy, using oral contraceptives or broad-spectrum antibiotics and those having Human Immunodeficiency Virus infection.Objective of the present study was to identify the various species of Candida isolated from patients with Vulvovaginal Candidiasis.Methods: A cross-sectional study was done from January to June 2018. A total of 69 clinically suspected VVC cases were included in the study whose high vaginal swabs were collected and subjected to direct microscopy and cultured on Sabouraud Dextrose Agar (SDA). Creamy white pigmented colonies on SDA were confirmed as Candida by doing Gram抯 staining. Speciation of positive cultures was confirmed by conventional methods like Corn Meal agar culture, Germ tube test (GTT), Candida CHROM Agar Medium (CAM), sugar assimilation and fermentation test.Results: Out of 69 clinically suspected VVC cases included in the study, only 14 cultures were positive for Candida species. All the 14 isolates were found to be Non-albicans Candida (NAC). Most common species isolated were Candida glabrata 7(50%), followed by Candida tropicalis 3(21.4%), Candida lusitaniae 3(21.4%), and Candida parapsilosis 1 (7.1%).Conclusions: Candida albicans was known to be the most common causative agent of VVC as it was isolated in 90% cases. However, this study showed that all isolates from VVC were Non-albicans Candida. Therefore, species identification should be done in all microbiology laboratories for accurate diagnosis of VVC.en_US
dc.identifier.affiliationsDepartment of Microbiology, Integral Institute of Medical Sciences and Research, Integral University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Medical Microbiology, Saaii College of Medical Science and Technology, Chaubepur, Kanpur, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Obstetrics and Gynaecology, 4Department of Community Medicine, Integral Institute of Medical Sciences and Research, Integral University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology, Hind Institute of Medical Sciences, Mau, Ataria, Sitapur, Uttar Pradesh, Indiaen_US
dc.identifier.citationJahan Noor, Vishnoi Pratishtha, Gupta Bhavana, Srivastava Swati, Ahmad Siraj, Khatoon Razia. Identification of various Candida species isolated from patients with vulvovaginal candidiasis attending a tertiary care hospital in North India. International Journal of Advances in Medicine. 2020 Feb; 7(2): 261-266en_US
dc.identifier.issn2349-3925
dc.identifier.issn2349-3933
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/194578
dc.languageenen_US
dc.publisherMedip Academyen_US
dc.relation.issuenumber2en_US
dc.relation.volume7en_US
dc.source.urihttps://dx.doi.org/10.18203/2349-3933.ijam20200076en_US
dc.subjectCandida CHROM agar mediumen_US
dc.subjectCorn meal agaren_US
dc.subjectNon-albicans candidaen_US
dc.subjectVulvovaginal candidiasisen_US
dc.titleIdentification of various Candida species isolated from patients with vulvovaginal candidiasis attending a tertiary care hospital in North Indiaen_US
dc.typeJournal Articleen_US
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