α/β Globin mRNA of β-thalassemia genes with premature termination

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dc.contributor.authorRatchanok Ngoupromminen_US
dc.contributor.authorNattaya Sae-ungen_US
dc.contributor.authorSupan Fucharoenen_US
dc.contributor.authorGoonnapa Fucharoenen_US
dc.contributor.authorKanokwan Sanchaisuriyaen_US
dc.date.accessioned2011-02-20T04:32:56Z
dc.date.available2011-02-20T04:32:56Z
dc.date.created2010-01-20en_US
dc.date.issued2010-01-20en_US
dc.description.abstractMutation of β-globin gene causing translation-premature termination results in significant decrease of the mRNA abundance. This phenomenon arises from the degradation of mutant mRNA by nonsense codon-mediated mRNA decay (NMD). In this study, α/β-globin mRNA ratio was determined by semi-quantitative RT-PCR in β-thalassemia carriers with the β17, β41/42, β71/72 and β27 mutations and in a patient with compound heterozygous β17, β27 mutations. Values were compared with normal individual. The α/β-globin mRNA ratio of normal individual was found to be 0.98 whereas those of heterozygous for β17, β41/42, β71/72 and compound heterozygous β17/β27 were 0.89 1.66 1.60 and 3.09, respectively. Direct DNA sequencing of the cDNA demonstrated mutant mRNA only in the carrier with β17 mutation but not other mutations. This result was in concordance with the α/β-globin mRNA ratio observed. This data indicates that the α/β-globin mRNA ratio is dependent on the type and the location of premature termination mutation and related to some other factors involving NMD mechanism in cells.en_US
dc.identifier.citationJournal of Medical Technology and Physical Therapy; Volume 19 Number 2, May - August 2007en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/130873
dc.language.isoen_USen_US
dc.publisherKhon Kaen Universityen_US
dc.rightsKhon Kaen Universityen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/article/view/601en_US
dc.titleα/β Globin mRNA of β-thalassemia genes with premature terminationen_US
dc.typeORIGINAL ARTICLEen_US
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