Further studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma.

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dc.contributor.authorRoche, Men_US
dc.contributor.authorPattabiraman, T Nen_US
dc.date.accessioned1992-04-01en_US
dc.date.accessioned2009-05-27T09:02:27Z
dc.date.available1992-04-01en_US
dc.date.available2009-05-27T09:02:27Z
dc.date.issued1992-04-01en_US
dc.description.abstractLoss of chymotrypsin binding capacity of alpha 2-macroglobulin in diabetic plasma on in vitro incubation, could be partially prevented by phenylmethyl sulphonyl fluoride and pepstatin A. Prior ten-fold dilution of plasma with 0.02 M phosphate buffer (pH 7.0) completely arrested the process. The phenomenon could not be reactivated by Ca2+, lecithin or bovine serum albumin. Diabetic plasma, like normal plasma, exhibited maximal hydrolytic activities on H-D-Pro-Phe-Arg-p-nitroanilide, H-D-Val-Leu-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide. The hydrolytic activities were not significantly diminished on incubation of plasma at 37 degrees C for 12 hr, unlike alpha 2-macroglobulin activity. On gel chromatography on Sephadex G-200, part of the proteolytic activity in diabetic plasma coeluted with alpha 2-macroglobulin in the VO region. A second activity peak (absent in normal plasma) was eluted with a Ve/V0 value of 1.40. Possible role of free proteinases in diabetic plasma in the inactivation of alpha 2-macroglobulin is discussed.en_US
dc.description.affiliationDepartment of Biochemistry, Kasturba Medical College, Manipal, Karnataka, India.en_US
dc.identifier.citationRoche M, Pattabiraman TN. Further studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma. Indian Journal of Biochemistry & Biophysics. 1992 Apr; 29(2): 189-91en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26254
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshChymotrypsin --metabolismen_US
dc.subject.meshDiabetes Mellitus --blooden_US
dc.subject.meshEndopeptidases --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligopeptidesen_US
dc.subject.meshProtease Inhibitors --pharmacologyen_US
dc.subject.meshSubstrate Specificityen_US
dc.subject.meshalpha-Macroglobulins --metabolismen_US
dc.titleFurther studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma.en_US
dc.typeJournal Articleen_US
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