Further studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma.
dc.contributor.author | Roche, M | en_US |
dc.contributor.author | Pattabiraman, T N | en_US |
dc.date.accessioned | 1992-04-01 | en_US |
dc.date.accessioned | 2009-05-27T09:02:27Z | |
dc.date.available | 1992-04-01 | en_US |
dc.date.available | 2009-05-27T09:02:27Z | |
dc.date.issued | 1992-04-01 | en_US |
dc.description.abstract | Loss of chymotrypsin binding capacity of alpha 2-macroglobulin in diabetic plasma on in vitro incubation, could be partially prevented by phenylmethyl sulphonyl fluoride and pepstatin A. Prior ten-fold dilution of plasma with 0.02 M phosphate buffer (pH 7.0) completely arrested the process. The phenomenon could not be reactivated by Ca2+, lecithin or bovine serum albumin. Diabetic plasma, like normal plasma, exhibited maximal hydrolytic activities on H-D-Pro-Phe-Arg-p-nitroanilide, H-D-Val-Leu-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide. The hydrolytic activities were not significantly diminished on incubation of plasma at 37 degrees C for 12 hr, unlike alpha 2-macroglobulin activity. On gel chromatography on Sephadex G-200, part of the proteolytic activity in diabetic plasma coeluted with alpha 2-macroglobulin in the VO region. A second activity peak (absent in normal plasma) was eluted with a Ve/V0 value of 1.40. Possible role of free proteinases in diabetic plasma in the inactivation of alpha 2-macroglobulin is discussed. | en_US |
dc.description.affiliation | Department of Biochemistry, Kasturba Medical College, Manipal, Karnataka, India. | en_US |
dc.identifier.citation | Roche M, Pattabiraman TN. Further studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma. Indian Journal of Biochemistry & Biophysics. 1992 Apr; 29(2): 189-91 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/26254 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.asp | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Chymotrypsin --metabolism | en_US |
dc.subject.mesh | Diabetes Mellitus --blood | en_US |
dc.subject.mesh | Endopeptidases --blood | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Oligopeptides | en_US |
dc.subject.mesh | Protease Inhibitors --pharmacology | en_US |
dc.subject.mesh | Substrate Specificity | en_US |
dc.subject.mesh | alpha-Macroglobulins --metabolism | en_US |
dc.title | Further studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma. | en_US |
dc.type | Journal Article | en_US |
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