Evaluation of Bithynia funiculata snail antigens by ELISA-serodiagnosis of human opisthorchiasis.

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dc.contributor.authorWatthanakulpanich, Den_US
dc.contributor.authorWaikagul, Jen_US
dc.contributor.authorAnantaphruti, M Ten_US
dc.contributor.authorDekumyoy, Pen_US
dc.date.accessioned2009-05-27T16:46:34Z
dc.date.available2009-05-27T16:46:34Z
dc.date.issued1997-09-30en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractFour batches of crude somatic antigens from: (1) Opisthorchis viverrini adult worms, (2) Bithynia funiculata-whole body, (3) B. funiculata-head-foot, and (4) B. funiculata-visceral mass were assayed against sera from 81 opisthorchiasis patients, 30 parasite-free healthy individuals, and 50 individuals infected with other helminthic infections, and their antibody levels determined. By IgG-ELISA, the antigenic reactive proteins were found in both the head-foot and the visceral mass of B. funiculata snails, but the whole snail antigens gave the best results. Furthermore, it was as good as when O. viverini antigens were used. Antibody levels of sera from patients with opisthorchiasis assayed against antigens from whole B. funiculata snails were significantly higher than those of the other two groups. The cut-off value for positivity at 0.228 gave 80.2% sensitivity and 81.2% specificity. Cross reactions were observed with sera from patients with paragonimiasis and strongyloidiasis. No cross reactions were found to occur with sera from healthy individuals.en_US
dc.description.affiliationDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationWatthanakulpanich D, Waikagul J, Anantaphruti MT, Dekumyoy P. Evaluation of Bithynia funiculata snail antigens by ELISA-serodiagnosis of human opisthorchiasis. The Southeast Asian Journal of Tropical Medicine and Public Health. 1997 Sep; 28(3): 593-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35984
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Helminth --analysisen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --analysisen_US
dc.subject.meshOpisthorchiasis --diagnosisen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSnails --immunologyen_US
dc.titleEvaluation of Bithynia funiculata snail antigens by ELISA-serodiagnosis of human opisthorchiasis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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