Molecular typing of mealybug Phenacoccus solenopsis populations from different hosts and locations in Punjab, India.
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Date
2012-05
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Abstract
True identity and existence of genetic variability in mealy bug from different regions holds immense significance
for adopting appropriate control measures along with predicting the development of any biotypes. Mealy bug,
Phenacoccus solenopsis adults were collected from four host plants i.e., Gossypium hirsutum – cotton (C),
Abelmoschus esculentus – okra (O), Pennisetum glaucum – Napier Bajra (B) and a weed – Parthenium
hysterophorus (P) in five cotton growing districts i.e., Abohar, Bathinda, Mansa, Muktsar and Faridkot of
Punjab state. Variability among different populations was investigated through comparative analysis of four
different RAPD markers. The genetic similarity dendrogram established that irrespective of the host plant and
the collection site, 20 mealybug populations were distinguishable into two major clades that were related to
each other by 68 %. Clade 1 included populations from Abohar district; it also included a single population
each from Muktsar and Bathinda districts. The populations from all the other districts were grouped under
Clade 2 with genetic similarity of 78 % Even under Clade 2, individual populations appeared to exist in
location specific sub-clades. Thus, there is great possibility of development of biotypes which may differ in
resistance to insecticides and host plant specificity.
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Keywords
Cotton, Genetic diversity, Mealy bug, Phenacoccus solenopsis
Citation
Singh Satnam, Sharma Rakesh, Kumar Rajinder, Gupta V K, Dilawari V K. Molecular typing of mealybug Phenacoccus solenopsis populations from different hosts and locations in Punjab, India. Journal of Environmental Biology. 2012 May; 33(3): 539-543.