Evaluation of a modified double-disc synergy test for detection of extended spectrum beta-lactamases in AMPC beta-lactamase-producing proteus mirabilis.

Simple item page
dc.contributor.authorKhan, M K Ren_US
dc.contributor.authorThukral, S Sen_US
dc.contributor.authorGaind, Ren_US
dc.date.accessioned2008-01-30en_US
dc.date.accessioned2009-05-28T09:28:27Z
dc.date.available2008-01-30en_US
dc.date.available2009-05-28T09:28:27Z
dc.date.issued2008-01-30en_US
dc.description.abstractThe detection of extended-spectrum beta-lactamases (ESBLs) in gram-negative bacteria that produce AmpC beta-lactamases is problematic. In the present study, the performance of modified double-disc synergy test (MDDST) that employs a combination of cefepime and piperacillin-tazobactam for the detection of Proteus mirabilis producing extended spectrum and AmpC beta-lactamases was evaluated and compared with double-disc synergy test (DDST) and NCCLS phenotypic disc confirmatory test (NCCLS-PDCT). A total of 90 clinical isolates of P. mirabilis , which met the CLSI (Clinical and Laboratory Standards Institute) screening criteria that these had broth microdilution (BMD) MIC of > or =2 mg/mL for at least one extended spectrum cephalosporin [ceftazidime (CAZ), cefotaxime (CTX) and cefpodoxime], were selected for the study. MDDST detected ESBLs in 40/90 of the isolates, whereas DDST detected ESBLs in only 25 isolates. NCCLS-PDCT could detect ESBLs in 39 isolates using CAZ and CAZ + clavulanic acid (CLA) combination, whereas CTX and CTX + CLA combination could detect only 37 isolates as ESBL positive. As many as 34/40 ESBL positive isolates were confirmed to be AmpC beta-lactamase positive by the modified three-dimensional test (MTDT). MDDST and NCCLS-PDCT could detect ESBLs in all the 34 AmpC positive isolates, whereas DDST could detect ESBLs in only 19 isolates. The study demonstrated that MDDST is superior to DDST and as sensitive as NCCLS-PDCT. However, MDDST seems to have enhanced potential for the detection of ESBLs in AmpC beta-lactamase-producing P. mirabilis .en_US
dc.description.affiliationDepartment of Microbiology, V P Chest Institute, University of Delhi, Delhi - 110 007, India.en_US
dc.identifier.citationKhan MK, Thukral SS, Gaind R. Evaluation of a modified double-disc synergy test for detection of extended spectrum beta-lactamases in AMPC beta-lactamase-producing proteus mirabilis. Indian Journal of Medical Microbiology. 2008 Jan-Mar; 26(1): 58-61en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/53977
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshAnti-Bacterial Agents --pharmacologyen_US
dc.subject.meshCephalosporins --pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Tests --methodsen_US
dc.subject.meshPenicillanic Acid --analogs & derivativesen_US
dc.subject.meshPiperacillin --pharmacologyen_US
dc.subject.meshProteus Infections --microbiologyen_US
dc.subject.meshProteus mirabilis --drug effectsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshbeta-Lactamases --analysisen_US
dc.titleEvaluation of a modified double-disc synergy test for detection of extended spectrum beta-lactamases in AMPC beta-lactamase-producing proteus mirabilis.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Medical Microbiology