Detection of antibodies against Salmonella typhi outer membrane protein (OMP) preparation in typhoid fever patients.

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dc.contributor.authorVerdugo-Rodriguez, Aen_US
dc.contributor.authorGam, L Hen_US
dc.contributor.authorDevi, Sen_US
dc.contributor.authorKoh, C Len_US
dc.contributor.authorPuthucheary, S Den_US
dc.contributor.authorCalva, Een_US
dc.contributor.authorPang, Ten_US
dc.date.accessioned2009-05-27T17:17:26Z
dc.date.available2009-05-27T17:17:26Z
dc.date.issued1993-06-01en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractAn indirect ELISA was used to detect antibodies against outer membrane protein preparations (OMPs) from Salmonella typhi. Sera from patients with a definitive diagnosis of typhoid fever (TF) gave a mean absorbance reading, at 414 nm, of 1.52 +/- 0.23 as compared to 0.30 +/- 0.11 for sera from healthy individuals. This gave a positive to negative ratio of absorbance readings of approximately 5.1. Suspected TF patients (no isolation of S. typhi), with positive and negative Widal titers had mean absorbance readings of 1.282 +/00.46 and 0.25 +/- 0.19, respectively. Sera from patients with leptospirosis, rickettsial typhus, dengue fever, and other infections gave mean absorbances of 0.20 +/- 0.08, 0.24 +/- 0.08, 0.27 +/- 0.08, and 0.31 +/- 0.16, respectively. The sensitivity, specificity, positive and negative predictive values were 100%, 94%, 80% and 100%, respectively. The antibody response detected in the definitive TF cases was predominantly IgG in nature and no cross-reactivity was seen with OMP preparations extracted from E. coli. Variable reactivity was noted with OMP preparations obtained from other Salmonella spp. Three major OMPs are presented in the antigen preparation and strong binding of positive sera was detected to all three bands.en_US
dc.description.affiliationInstitute for Advanced Studies, University of Malaya, Kuala Lumpur.en_US
dc.identifier.citationVerdugo-Rodriguez A, Gam LH, Devi S, Koh CL, Puthucheary SD, Calva E, Pang T. Detection of antibodies against Salmonella typhi outer membrane protein (OMP) preparation in typhoid fever patients. Asian Pacific Journal of Allergy and Immunology. 1993 Jun; 11(1): 45-52en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36868
dc.language.isoengen_US
dc.subject.meshAntibodies, Bacterial --analysisen_US
dc.subject.meshBacterial Infections --immunologyen_US
dc.subject.meshBacterial Outer Membrane Proteins --immunologyen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshEscherichia coli --immunologyen_US
dc.subject.meshFalse Positive Reactionsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoglobulin G --immunologyen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshSalmonella typhi --immunologyen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshTyphoid Fever --diagnosisen_US
dc.titleDetection of antibodies against Salmonella typhi outer membrane protein (OMP) preparation in typhoid fever patients.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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