Molecular characterization of hepatitis A virus from children hospitalized at a tertiary care centre in northwest India

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dc.contributor.authorMalhotra, Ben_US
dc.contributor.authorKanwar, Aen_US
dc.contributor.authorReddy, PVen_US
dc.contributor.authorChauhan, Aen_US
dc.contributor.authorTiwari, Jen_US
dc.contributor.authorBhargava, Sen_US
dc.contributor.authorVerma, H Nen_US
dc.date.accessioned2020-04-10T01:38:30Z
dc.date.available2020-04-10T01:38:30Z
dc.date.issued2018-05
dc.description.abstractBackground & objectives: Hepatitis A virus (HAV) infection is a major cause of childhood hepatitis, prevalent worldwide. HAV is classified into seven genotypes I-VII; genotypes III and I are the most common among humans. The present work was carried out to identify the genotypes prevalent in children suspected to have acute viral hepatitis (AVH), hospitalized at a tertiary care centre in northwest India. Methods: A total of 1269 blood samples from children (0-15 yr of age) clinically suspected of viral hepatitis were screened for anti-HAV IgM. Acute phase serum was processed for RNA extraction and amplified by nested polymerase chain reaction (PCR) followed by sequencing of representative samples. Results: Among the 1269 samples tested, 642 (50.59%) were positive for anti-HAV IgM; among the positive samples, 171 patients having a history of less than seven days were tested by PCR, of whom 141 (82.45%) were found to be PCR positive. Nucleotide sequencing of a representative 44 samples showed high homology; all the samples were found to be of genotype IIIA. Interpretation & conclusions: Hepatitis A was prevalent during July to September and in predominantly children less than five years age. Only genotype IIIA was detected in all the samples.en_US
dc.identifier.affiliationsAdvanced Basic Sciences & Clinical Research Laboratory, Department of Microbiology & Immunology, Sawai Man Singh Medical College, Jaipur, Indiaen_US
dc.identifier.affiliationsSchool of Life & Basic Sciences, Jaipur National University, Jaipur, Indiaen_US
dc.identifier.citationMalhotra B, Kanwar A, Reddy PV, Chauhan A, Tiwari J, Bhargava S, Verma H N. Molecular characterization of hepatitis A virus from children hospitalized at a tertiary care centre in northwest India. Indian Journal of Medical Research. 2018 May; 147(5): 507-512en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195533
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber5en_US
dc.relation.volume147en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1910_15en_US
dc.subjectGenotypingen_US
dc.subjectHAVen_US
dc.subjecthepatitis Aen_US
dc.subjectphylogenetic analysisen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectserologyen_US
dc.titleMolecular characterization of hepatitis A virus from children hospitalized at a tertiary care centre in northwest Indiaen_US
dc.typeJournal Articleen_US
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