In vitro studies on inhibitory effect of proanthocyanidins in modulation of neutrophils and macrophages.

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dc.contributor.authorGovindaraj, Jayamathi
dc.contributor.authorEmmadi, Pamela
dc.contributor.authorPuvanakrishnan, Rengarajulu
dc.date.accessioned2011-11-14T06:07:32Z
dc.date.available2011-11-14T06:07:32Z
dc.date.issued2010-06
dc.description.abstractThe role of proanthocyanidins (PC), a novel flavonoid extracted from grape seeds was studied in vitro in the modulation of neutrophil and macrophage function. We attempted to assess the levels of non-enzymatic and enzymatic mediators in the presence or absence of PC in 4-phorbol-12--myristate-13-acetate (PMA)-stimulated neutrophils isolated from humans and rats, E. coli endotoxin-stimulated macrophages and macrophages isolated from E. coli endotoxin-induced experimental periodontitis in rats. Addition of PC at a concentration of 50 µg/ml effectively blocked the release of reactive oxygen species (ROS) and reactive nitrogen species (RNS) and exhibited a marked inhibition of myeloperoxidase (MPO) and lysosomal enzymes (p<0.001), as compared to PMA-stimulated neutrophils (human and rats) and neutrophils isolated from experimental periodontitis in rats. The levels of ROS, RNS and lysosomal enzymes were found to be elevated (p<0.001) and addition of PC significantly (p<0.001) reduced these levels as compared to those from E. coli endotoxin-stimulatedmacrophages from rats and macrophages isolated from experimental periodontitis in rats (p<0.001). Thus, the study demonstrated that PC decreased the levels of ROS and RNS and also inhibited the MPO and lysosomal enzymes activities in experimental periodontitis in rats. In addition, this study clearly indicated that PC could be developed as an effective antiinflammatory agent.en_US
dc.identifier.citationGovindaraj Jayamathi, Emmadi Pamela, Puvanakrishnan Rengarajulu. In vitro studies on inhibitory effect of proanthocyanidins in modulation of neutrophils and macrophages. Indian Journal of Biochemistry & Biophysics. 2010 June; 47(3): 141-147.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135258
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/9810/1/IJBB%2047%283%29%20141-147.pdfen_US
dc.subjectLysosomal enzymesen_US
dc.subjectMacrophagesen_US
dc.subjectNeutrophilsen_US
dc.subjectProanthocyanidinsen_US
dc.subjectReactive nitrogen speciesen_US
dc.subjectReactive oxygen speciesen_US
dc.subject.meshAnimals
dc.subject.meshAnti-Inflammatory Agents, Non-Steroidal --pharmacology
dc.subject.meshHumans
dc.subject.meshLysosomes --drug effects
dc.subject.meshLysosomes --enzymology
dc.subject.meshMacrophages, Peritoneal --drug effects
dc.subject.meshMacrophages, Peritoneal --metabolism
dc.subject.meshMale
dc.subject.meshNeutrophils --drug effects
dc.subject.meshNeutrophils --metabolism
dc.subject.meshPeriodontitis --drug therapy
dc.subject.meshPeriodontitis --metabolism
dc.subject.meshPeroxidase --antagonists & inhibitors
dc.subject.meshProanthocyanidins --pharmacology
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshReactive Nitrogen Species --metabolism
dc.subject.meshReactive Oxygen Species --metabolism
dc.titleIn vitro studies on inhibitory effect of proanthocyanidins in modulation of neutrophils and macrophages.en_US
dc.typeArticleen_US
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