Determination of HER-2/neu Gene Amplification in Breast Cancer Using Real-Time Quantitative PCR and Comparison with Immunohistochemical and Chromogenic in-situ Hybridization Results

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dc.contributor.authorPreecha Ruangvejvorachaien_US
dc.contributor.authorSunanta Chariyalertsaken_US
dc.contributor.authorWichai Purisaen_US
dc.contributor.authorSongkhun Vinyuvaten_US
dc.date.accessioned2011-02-22T08:15:50Z
dc.date.available2011-02-22T08:15:50Z
dc.date.created2011-02-03en_US
dc.date.issued2011-02-03en_US
dc.description.abstractHER-2/neu status is used as a marker for Herceptin\® therapy. The aim of this study was to evaluate the usefulness of quantitative real-time PCR (qPCR) in determining the HER-2/neu amplification status of breast cancer, by comparing qPCR, CISH, and chromogenic in-situ hybridization (CISH) and immunohistochemistry (IHC) results from the same samples. Thirty breast carcinomas were examined using the three methods. Twenty-two tumors were 2+ or 3+ positive with IHC, while eight samples were either completely negative or 1+. Fifteen positive and twelve negative cases were detected by both CISH and qPCR, which led to similar results. Comparison of CISH and qPCR together with IHC showed that qPCR was more sensitive in detecting HER-2/neu gene amplification in tumors scored as 2+ with IHC. In conclusion, qPCR may serve as useful alternative to CISH for detecting HER-2/neu gene amplification in breast-cancer patients. (Thai Cancer J 2010;30:104-111)en_US
dc.identifier.citationThai Cancer Journal; Vol.30 No.3 July-September 2010; 104-111en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/132391
dc.language.isoen_USen_US
dc.publisherThai Cancer Journalen_US
dc.rightsNational Cancer Institute of Thailand, Bangkok, Thailanden_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/TCJ/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/TCJ/article/view/5138en_US
dc.titleDetermination of HER-2/neu Gene Amplification in Breast Cancer Using Real-Time Quantitative PCR and Comparison with Immunohistochemical and Chromogenic in-situ Hybridization Resultsen_US
dc.typeResearch Articlesen_US
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