Use of Cellulase enzyme obtained from Monilia (Moniliophthora roreri) for treatment of solid waste of Cob, Rice husks and Cocoa shell.

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dc.contributor.authorSolís, Nataly
dc.contributor.authorChiriboga, Carlos
dc.contributor.authorÁvalos, Rodrigo
dc.contributor.authorRueda, Darwin
dc.contributor.authorAlbán, Cristina
dc.contributor.authorManjunatha, Bangeppagari
dc.contributor.authorRueda, Bryan
dc.contributor.authorSelvanayagam, Mariadoss
dc.date.accessioned2016-09-05T09:27:51Z
dc.date.available2016-09-05T09:27:51Z
dc.date.issued2016-03
dc.description.abstractEcuador generates lots of lingo cellulosic organic waste from cocoa, rice and corn agribusiness, and these products are rich in cellulose and can be used in fermentation processes by enzyme producing yeasts. In this study, the phytopathogenic fungus Moniliophthora roreri was isolated, characterized and the kinetics of production of cellulose was studied using rice husks, cocoa shell and cob as a growth inducer substance for a period of 20 days. The carboxymethylcellulose (CMC) was used as specific substrate to evaluate the kinetic parameters of the enzyme at different concentration of 0.5%, 1% and 1.5%; to evaluate the Vmax, Km, specific activity, Kcat we worked with the Michaelis-Menten and Line weaver-Burk linearization and the crude enzyme was purified with ammonium sulfate. The enzyme production was increased on 10th, 15th and 20th day in rice husk, cocoa shell and cob, respectively. There was also an increased enzyme activity and the kinetic parameters obtained from rice husk. The purification is a step to increase the specific activity of the enzyme in each of the extracts is under study.en_US
dc.identifier.citationSolís Nataly, Chiriboga Carlos, Ávalos Rodrigo, Rueda Darwin, Albán Cristina, Manjunatha Bangeppagari, Rueda Bryan, Selvanayagam Mariadoss. Use of Cellulase enzyme obtained from Monilia (Moniliophthora roreri) for treatment of solid waste of Cob, Rice husks and Cocoa shell. Journal of Applied Pharmaceutical Science. 2016 Mar; 6(3): 66-70.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/176941
dc.language.isoenen_US
dc.source.urihttps://www.japsonline.com/abstract.php?article_id=1804en_US
dc.subjectMoniliophthora rorerien_US
dc.subjectCelluloseen_US
dc.subjectEnzyme activityen_US
dc.subjectEnzyme purificationen_US
dc.subjectKinetic parametersen_US
dc.titleUse of Cellulase enzyme obtained from Monilia (Moniliophthora roreri) for treatment of solid waste of Cob, Rice husks and Cocoa shell.en_US
dc.typeArticleen_US
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