In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae).

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dc.contributor.authorSukontason, Ken_US
dc.contributor.authorBoonsriwong, Nen_US
dc.contributor.authorChaithong, Uen_US
dc.contributor.authorSukontason, K Len_US
dc.contributor.authorPiangjai, Sen_US
dc.date.accessioned2009-05-27T15:59:32Z
dc.date.available2009-05-27T15:59:32Z
dc.date.issued2001-03-04en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe effects of trypsin, bile extract, temperature and acid-based condition for the in vitro excystation of Haplorchis taichui metacercariae were studied. At 37 degrees C, approximately half the number of metacercariae excysted when exposed to 1% trypsin for 15 minutes with no more excystation found beyond this time. Increasing trypsin concentration seemed to reduce the excystation rate while bile extract was, however, unlikely to be an absolute requirement. A temperature of 37 degrees-41 degrees C yielded a similar excystation result in combination with 1% trypsin; however, less excystation occurred at a lower temperature of 35 degrees C. The acid-based environment of pH 8 gave the best excystation result in association with 1% trypsin at a temperature of 39 degrees C. Higher and lower basicity produced a smaller excystation rate. An environmental condition of 1% trypsin at pH 8 and a temperature of between 37 degrees-41 degrees C was recommended for the in vitro excystation of H. taichui metacercariae. The relatively broad temperature and pH range condition for the excystation of H. taichui corresponded with various definitive hosts that were infected naturally by this fluke.en_US
dc.description.affiliationDepartment of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand. ksukonta@mail.med.cmu.ac.then_US
dc.identifier.citationSukontason K, Boonsriwong N, Chaithong U, Sukontason KL, Piangjai S. In vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae). The Southeast Asian Journal of Tropical Medicine and Public Health. 2001 ; 32 Suppl 2(): 27-30en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34156
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBileen_US
dc.subject.meshCyprinidae --parasitologyen_US
dc.subject.meshFish Diseases --parasitologyen_US
dc.subject.meshHeterophyidae --physiologyen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshMuscle, Skeletal --parasitologyen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTrematode Infections --parasitologyen_US
dc.subject.meshTrypsinen_US
dc.titleIn vitro excystation of Haplorchis taichui (Trematoda: Heterophyidae).en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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