Bacterial and fungal keratitis in Upper Egypt: In vitro screening of enzymes, toxins and antifungal activity.

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dc.contributor.authorGharamah, Abdullah A
dc.contributor.authorMoharram, Ahmed M
dc.contributor.authorIsmail, Mady A
dc.contributor.authorAL-Hussaini, Ashraf K
dc.date.accessioned2015-01-28T05:36:49Z
dc.date.available2015-01-28T05:36:49Z
dc.date.issued2014-02
dc.description.abstractPurpose: This work was conducted to study the ability of bacterial and fungal isolates from keratitis cases in Upper Egypt to produce enzymes, toxins, and to test the isolated fungal species sensitivity to some therapeutic agents. Materials and Methods: One hundred and fifteen patients clinically diagnosed to have microbial keratitis were investigated. From these cases, 37 bacterial isolates and 25 fungal isolates were screened for their ability to produce extra‑cellular enzymes in solid media. In addition, the ability of fungal isolates to produce mycotoxins and their sensitivity to 4 antifungal agents were tested. Results: Protease, lipase, hemolysins, urease, phosphatase, and catalase were detected respectively in 48.65%, 37.84%, 59.46%, 43.24%, 67.57%, and 100% out of 37 bacterial isolates tested. Out of 25 fungal isolates tested during the present study, 80% were positive for protease, 84% for lipase and urease, 28% for blood hemolysis, and 100% for phosphatase and catalase enzymes. Thirteen fungal isolates were able to produce detectable amounts of 7 mycotoxins in culture medium (aflatoxins (B1, B2, G1, and G2), sterigmatocystin, fumagillin, diacetoxyscirpenol, zearalenone, T‑2 toxin, and trichodermin). Among the antifungal agents tested in this study, terbinafine showed the highest effect against most isolates in vitro. Conclusion: In conclusion, the ability of bacterial and fungal isolates to produce extracellular enzymes and toxins may be aid in the invasion and destruction of eye tissues, which, in turn, lead to vision loss.en_US
dc.identifier.citationGharamah Abdullah A, Moharram Ahmed M, Ismail Mady A, AL-Hussaini Ashraf K. Bacterial and fungal keratitis in Upper Egypt: In vitro screening of enzymes, toxins and antifungal activity. Indian Journal of Ophthalmology. 2014 Feb ; 62 (2): 196-203.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/155534
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005237/en_US
dc.subjectBacterialen_US
dc.subjectextracellular enzymesen_US
dc.subjectfungalen_US
dc.subjectkeratitisen_US
dc.subjecttoxinsen_US
dc.titleBacterial and fungal keratitis in Upper Egypt: In vitro screening of enzymes, toxins and antifungal activity.en_US
dc.typeArticleen_US
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