Role of RT-PCR and FISH in diagnosis and monitoring of acute promyelocytic leukemia.

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dc.contributor.authorPolampalli, S
dc.contributor.authorChoughule, A
dc.contributor.authorPrabhash, K
dc.contributor.authorAmare, P
dc.contributor.authorBaisane, C
dc.contributor.authorKabre, S
dc.contributor.authorMahadik, S
dc.contributor.authorShinde, S
dc.contributor.authorNair, R
dc.contributor.authorBanavali, S
dc.date.accessioned2013-01-16T10:35:15Z
dc.date.available2013-01-16T10:35:15Z
dc.date.issued2011-01
dc.description.abstractBackground: Patients with a presence of Promyelocytic Leukemia-Retinoic Acid Receptor Alpha (PML-RARA) genes rearrangement predict a favorable response to all-trans retinoic acid (ATRA), and a significant improvement in survival. Therefore, establishing the presence of PML-RARA rearrangement is important for optimal patient management. Aim: The objective of this study is to compare and assess the role of fluorescent in situ hybridization (FISH) and reverse transcriptase polymerase chain reaction (RT-PCR) in the diagnosis and long-term monitoring of Acute Promyelocytic Leukemia (APL). Materials and Methods: We compared 145 samples received at different interval of times to analyze the sensitivity of RT-PCR and FISH. Results: The failure rate for RT-PCR was 4% at baseline, 13% at induction, and 0% at the end of consolidation. And for FISH it was 8% at baseline, 38% at induction, and 66% at the end of consolidation. The predictive values of relapse in the patients who were positive and negative by RT-PCR, at the end of induction, were 60 % and 3%, respectively, and at end of consolidation it was 67 % and 4%, respectively. On the other hand the predictive values of relapse in patients who were positive and negative by FISH at end of induction were 57 % and 6%, respectively; while at end of consolidation it was 14% who were negative by FISH. Conclusion: Both RT-PCR and FISH are important for the diagnosis of APL cases, as both techniques complement each other in the absence or failure of any one of them. However, RT-PCR is more sensitive than FISH for the detection of minimal residual disease in the long-term monitoring of these patients. The present study shows that the predictive value of relapse is more associated with minimal residual disease (MRD) results by RT-PCR than that by FISH.en_US
dc.identifier.citationPolampalli S, Choughule A, Prabhash K, Amare P, Baisane C, Kabre S, Mahadik S, Shinde S, Nair R, Banavali S. Role of RT-PCR and FISH in diagnosis and monitoring of acute promyelocytic leukemia. Indian Journal of Cancer. 2011 Jan-Mar; 48(1): 60-67.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/144413
dc.language.isoenen_US
dc.source.urihttps://www.indianjcancer.com/article.asp?issn=0019-509X;year=2011;volume=48;issue=1;spage=60;epage=67;aulast=Polampallien_US
dc.subjectReverse transcriptase polymerase chain reactionen_US
dc.subjectfluorescent in situ hybridizationen_US
dc.subjectacute promyelocytic leukemiaen_US
dc.subject.meshAntineoplastic Agents --therapeutic use
dc.subject.meshFollow-Up Studies
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization, Fluorescence
dc.subject.meshLeukemia, Promyelocytic, Acute --diagnosis
dc.subject.meshLeukemia, Promyelocytic, Acute --drug therapy
dc.subject.meshLeukemia, Promyelocytic, Acute --genetics
dc.subject.meshNeoplasm, Residual --diagnosis
dc.subject.meshNeoplasm, Residual --drug therapy
dc.subject.meshNeoplasm, Residual --genetics
dc.subject.meshPrognosis
dc.subject.meshRNA, Messenger --genetics
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshTreatment Outcome
dc.subject.meshTretinoin --therapeutic use
dc.titleRole of RT-PCR and FISH in diagnosis and monitoring of acute promyelocytic leukemia.en_US
dc.typeArticleen_US
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