The Influence of Agitation Rates, pH and Calcium Carbonate on L-lysine Production by Bacillus subtilis using Agricultural Products as Carbon and Nitrogen Sources

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dc.contributor.authorOkpalla, Jen_US
dc.contributor.authorEkwealor, I. A.en_US
dc.date.accessioned2023-06-27T06:26:11Z
dc.date.available2023-06-27T06:26:11Z
dc.date.issued2022-12
dc.description.abstractL-Lysine is an essential amino acid that is required in the diet of humans and animals. It is utilized in human medicine, cosmetics and pharmaceutical industry. ’The influence of agitation rates, pH and calcium carbonate on L-lysine production by Bacillus subtilis using agricultural products as carbon and nitrogen sources was studied. The L-lysine-producing bacteria had already been isolated from Nigerian soil. They were purified and Identified as B. subtilis PR13 and B. subtilis PR9, using cultural, biochemical and molecular characteristics. Optimization of some parameters which included agitation rates, pH values and CaCO3 concentrations, on L-lysine production by the Bacillus species was carried out. The L-lysine was produced in 250 ml flasks containing fermentation media (FM1 and FM2). The findings revealed that, enhanced L-lysine yield of 2.10 and 1.33 mg/ml was observed at agitation rate of 180 rpm for B. subtilis PR13 and PR9 respectively. There was a positive correlation between agitation rates and L- lysine production by B. subtilis PR13 and PR9 (r = 0.96 and 0.83 respectively). The pH of 7.5, stimulated optimum L- lysine yield of 2.27 mg/ml for PR13 and 1.38 mg/ml for PR9. There was a positive correlation between pH values and L-lysine production by B. subtilis PR13 and PR 9 (r = 0.63 and 0.50 respectively). The supplementation of 40g/l of CaCO3, enhanced optimum L-lysine yield of 2.18 mg/ml for B. subtilis PR 13 and 1.30 mg/ml for B. subtilis PR9. There was a positive correlation between varying concentrations of calcium carbonate and L-lysine production by the B. subtilis PR13 (r =0.35), while negative correlation was observed for B. subtilis PR 9 (r = -0.10). The results obtained in the study illustrated that the optimization of process parameters could increase the L-lysine yield from agricultural products by B. subtilis PR13 and B. subtilis PR9.en_US
dc.identifier.affiliationsDepartment of Microbiology, Chukwuemeka Odumegwu Ojukwu University, P.M.B. 02 Uli, Anambra State, Nigeriaen_US
dc.identifier.affiliationsDepartment of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.en_US
dc.identifier.citationOkpalla J, Ekwealor I. A.. The Influence of Agitation Rates, pH and Calcium Carbonate on L-lysine Production by Bacillus subtilis using Agricultural Products as Carbon and Nitrogen Sources. Sciencedomain International. 2022 Dec; 32(11-12): 1-11en_US
dc.identifier.issn2456-7043
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/217186
dc.languageenen_US
dc.publisherMicrobiology Research Journal Internationalen_US
dc.relation.issuenumber11-12en_US
dc.relation.volume32en_US
dc.source.urihttps://doi.org/10.9734/mrji/2022/v32i111351en_US
dc.subjectBacillus speciesen_US
dc.subjectL-lysineen_US
dc.subjectsubmerged fermentationen_US
dc.subjectagitation rateen_US
dc.subjectpHen_US
dc.titleThe Influence of Agitation Rates, pH and Calcium Carbonate on L-lysine Production by Bacillus subtilis using Agricultural Products as Carbon and Nitrogen Sourcesen_US
dc.typeJournal Articleen_US
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