Magnesium induced vascular relaxation and role of Calcium-dependent K+ Channels

Simple item page
dc.contributor.authorUpadhyay-Dhungel, Ken_US
dc.contributor.authorKim, CJen_US
dc.contributor.authorDhungel, Aen_US
dc.date.accessioned2019-11-16T08:27:38Z
dc.date.available2019-11-16T08:27:38Z
dc.date.issued2013-03
dc.description.abstractBackground and objectives: Magnesium is established as a neuro-protective agent and now also known as a vasodilator. It has been known for treating vasospasm following subarachnoid hemorrhage. However, its action mechanism in cerebral vascular relaxation is not clear. Potassium channels play a pivotal role in the relaxation of smooth muscle cells. To investigate their role in magnesium-induced relaxation of basilar smooth muscle cells, we examined the effect of magnesium on potassium channels using the patch clamp technique on cells from rabbit basilar artery. Material and Methods: Fresh smooth muscle cells were isolated from the basilar artery by enzyme treatment. Whole cell current recording was done using patch-clamp technique. Appropriate bath solution was used to have potassium current. The effect of Magnesium was observed and to identify the potassium (K+) channel involved in the magnesium-induced currents, different potassium channel blockers were used. Results: Magnesium increased the step pulse-induced outward K+ currents by more than fortyfive percent over control level (p<0.01). The outward K+ current was decreased significantly by application of tetraethylammonium, a non-specific K+ channel blocker, and by iberiotoxin, a largeconductance Ca2+-activated K+ (BKCa) channel blocker, but was not inhibited by glibenclamide an ATP-sensitive K+ (KATP) channel blocker. Magnesium failed to increase the outward K+ currents in the presence of IBX. Conclusion: These results demonstrate that calcium dependent pottassium (BKCa) channels has role in magnesium induced vascular relaxation in rabbit basilar smooth muscle cells and needs to be worked out for human.en_US
dc.identifier.affiliationsDepartment of Neurosurgery, Chonbuk National University Medical School, South Korea and Associate Professor, Department of Physiology, Janaki Medical College, Janakpurdhamen_US
dc.identifier.affiliationsDepartment of Neurosurgery, Chonbuk National University Medical Schoolen_US
dc.identifier.affiliationsDepartment of Internal Medicine, King Edward Medical University, Lahoreen_US
dc.identifier.citationUpadhyay-Dhungel K, Kim CJ, Dhungel A. Magnesium induced vascular relaxation and role of Calcium-dependent K+ Channels. Janaki Medical College Journal of Medical Science. 2013 Mar; 1(1): 9-13en_US
dc.identifier.issn2091-2242
dc.identifier.issn2091-2358
dc.identifier.placeNepalen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/184625
dc.languageenen_US
dc.publisherJanaki Medical Collegeen_US
dc.relation.issuenumber1en_US
dc.relation.volume1en_US
dc.source.urihttps://doi.org/10.3126/jmcjms.v1i1.7880en_US
dc.subjectElectrophysiologyen_US
dc.subjectMagnesiumen_US
dc.subjectPatch Clampen_US
dc.subjectVasoconstrictionen_US
dc.titleMagnesium induced vascular relaxation and role of Calcium-dependent K+ Channelsen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jmcjms2013v1n1p9.pdf
Size:
133.55 KB
Format:
Adobe Portable Document Format
Download
Collections
Janaki Medical College Journal of Medical Science