Identification and characterization of bacteria isolated under selective pressure of volatile organic compounds.
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Date
2009-01
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Abstract
Several bacterial strains tolerant to volatile organic compounds (VOCs) were isolated from the air emissions of a typical industrial
wood painting plant. Four strains that grew vigorously on a mineral salt basal medium and VOC-saturated atmosphere were identified using
BIOLOG and fatty acids analysis. A 16S rDNA sequence comparison was also performed on three of the four strains. Their phylogenetic
positions were respectively: MC8M6 of the genus Arthrobacter, MCEP3 of the genus Microbacterium, MCEPFL2 of the genus Rhodococcus.
The fourth strain, C14, was identified as Pseudomonas aeruginosa. All the four strains showed multiple carbon sources among toluene,
butyl acetate, ethoxy propyl acetate, isobutyl alcohol, ethyl acetate, diaceton alcohol, methyl ethyl ketone, methyl isobutyl ketone. By
identifying a particular strain, an evaluation of its risk group can be made by comparing it to each strain type within important world-wide
culture collections. This is the first step required to implement correct procedures using the strains and in implementing the appropriate
biosafety containment in large scale applications.
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Keywords
Bacteria, Volatile organic compounds, 16S rRNA, Fatty acids, BIOLOG, Biofiltration
Citation
Civilini Marcello. Identification and characterization of bacteria isolated under selective pressure of volatile organic compounds. Journal of Environmental Biology. 2009 Jan; 30(1): 99-105.