Effect of exogenous H2O2 on antioxidant enzymes of Brassica juncea L. seedlings in relation to 24-epibrassinolide under chilling stress.

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dc.contributor.authorKumar, Manish
dc.contributor.authorSirhindi, Geetika
dc.contributor.authorBhardwaj, Renu
dc.contributor.authorKumar, Sandeep
dc.contributor.authorJain, Gagandeep
dc.date.accessioned2011-11-15T05:29:30Z
dc.date.available2011-11-15T05:29:30Z
dc.date.issued2010-12
dc.description.abstractHydrogen peroxide is most stable molecule among reactive oxygen species, which play a vital role in growth and development of plant as signaling molecule at low concentration in response to various abiotic and biotic stresses. Exogenous application of H2O2 is known to induce chilling tolerance in plants. Brassinosteroids are plant steroid hormones known for their anti-stress properties. In this study, effect of exogenous H2O2 on antioxidant defense system of Brassica juncea L. seedlings was investigated in 24-epibrassinolide (24-EBL) treated and untreated seedlings under chilling stress. The surface sterilized seeds of B. juncea L. were germinated in petriplates containing different concentrations of H2O2 alone and in combination with 10-8 M 24-EBL. Chilling treatment (4 ºC) was given to 10-days old seedlings grown in different treatments for 6 h daily up to 3 days. 24 h recovery period was given to chilling treated seedlings by placing at 25ºC ± 2ºC and harvested for antioxidant enzymes on 14th day after sowing (DAS). Treatment of 24-EBL in combination with H2O2 (15 and 20 mM) helped in reducing the toxicity of seed and seedlings due to H2O2 exposure on their germination rate, shoot and root length respectively. 24-EBL treatment at seed and seedling stage helped in alleviating the toxic effect of H2O2 through antioxidant defense system by increasing the activities of various enzymes involved in antioxidant defense system such as catalase (CAT, E.C. 1.11.1.6), ascorbate peroxidase (APOX, E.C. 1.11.1.11), and superoxide dismutase (SOD, E.C. 1.15.1.1). In conclusion, exogenous pretreatment of H2O2 to seeds of B. juncea L. adapted the seedlings to tolerate chilling stress, which was further ameliorated in combination of H2O2 with 24-EBL.en_US
dc.identifier.citationKumar Manish, Sirhindi Geetika, Bhardwaj Renu, Kumar Sandeep, Jain Gagandeep. Effect of exogenous H2O2 on antioxidant enzymes of Brassica juncea L. seedlings in relation to 24-epibrassinolide under chilling stress. Indian Journal of Biochemistry & Biophysics. 2010 Dec; 47(6): 378-382.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135291
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/10862/1/IJBB%2047%286%29%20378-382.pdfen_US
dc.subjectAntioxidantsen_US
dc.subjectROSen_US
dc.subjectH2O2en_US
dc.subjectBrassinosteroidsen_US
dc.subject24-Epibrassinolideen_US
dc.subjectBrassica juncea L.en_US
dc.subjectChilling stressen_US
dc.subject.meshAcclimatization --drug effects
dc.subject.meshAcclimatization --physiology
dc.subject.meshAntioxidants --metabolism
dc.subject.meshAscorbate Peroxidases
dc.subject.meshBrassinosteroids
dc.subject.meshCatalase --metabolism
dc.subject.meshCholestanols --pharmacology
dc.subject.meshCold Temperature
dc.subject.meshHydrogen Peroxide --pharmacology
dc.subject.meshHydrogen Peroxide --toxicity
dc.subject.meshMustard Plant --drug effects
dc.subject.meshMustard Plant --enzymology
dc.subject.meshPeroxidases --metabolism
dc.subject.meshSeedling --drug effects
dc.subject.meshSeedling --enzymology
dc.subject.meshSteroids, Heterocyclic --pharmacology
dc.subject.meshSuperoxide Dismutase --metabolism
dc.titleEffect of exogenous H2O2 on antioxidant enzymes of Brassica juncea L. seedlings in relation to 24-epibrassinolide under chilling stress.en_US
dc.typeArticleen_US
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